Table 1 Germline usage analysis for single plasmablast-derived antigen-specific mAbs
Antibody ID | Serotype(s) | VH | Â | Â | DH | JH | VL | Â | Â | JL |
|---|---|---|---|---|---|---|---|---|---|---|
Family | CDR3 Length | HCDR3 AA sequence | Family | Family | Family | CDR3 length | LCDR3 AA sequence | Family | ||
1A4 | 1 | 3–23*04 | 10 | DVRGSGSNSY | 3–10*01 | 4*02 | K2–30*01 | 9 | MQGTYWPPIT | KJ2*01 |
1C6 | 1 | 3–53*01 | 8 | EVDYAFDP | 3–16*01 | 5*02 | L8–61*01 | 10 | VLFMGSGTWV | LJ3*02 |
1D4 | 1 | 3–74*03 | 8 | SASGWYVN | 6–19*01 | 4*02 | K4–1*01 | 9 | QHYASVPWT | KJ1*01 |
1A6 6 total | 4 | 3–30-3*01 | 10 | DPDTSNKIDY | 2–2*02 | 4*02 | K2–30*01 | 10 | MQGTYWPPIT | KJ5*01 |
1A10 | 4 | 3–7*01 | 10 | RMFGSSFRDY | 6–6*01 | 4*02 | L2–14*01 | 10 | NSYTSSKTWV | LJ3*02 |
1B1 | 4 | 1–46*01 | 16 | GGLLPGVAGATSPFQH | 2–2*01 | 1*01 | K3–20*01 | 9 | QRYGSSPVT | KJ4*01 |
1C3 | 4 | 3–23*01 | 12 | GPVLPAPKEFDY | 2–15*01 | 4*02 | L4–69*01 | 10 | QTWDTVTNWV | LJ3*02 |
1C4 2 total | 6A, 6B | 3–7*03 | 8 | EEWYRFDY | 3–3*02 | 4*02 | L2–8*01 | 10 | SSHAGSKNVI | LJ2*01 |
1D7 | 6A, 6B | 3–7*01 | 8 | EIWFREDY | 3–10*01 | 4*02 | L2–8*01 | 10 | GSRVGSNSVV | LJ2*01 |
1A2 | 7F | 3–33*01 | 14 | EPRAIADNYYGMDV | 3–3*01 | 6*02 | L10–54*01 | 11 | SAWDSSLNAWV | LJ3*02 |
1B2 2 total | 18C | 3–7*01 | 9 | LGGWRHLDY | 3–16*02 | 4*02 | K1–39*01 | 9 | QQSYSSPYT | KJ2*01 |