Table 1 List of primers used for bacterial identification and detection of MSCRAMMs genes (fnbA, fnbB and cna), biofim control genes (icaA and icaD) and exoenzymes genes (sspA, sspB and geh)
Target gene | Sequence (5’-3’) | aT (°C) | Amplified fragment | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
Sa442 | 5’-AATCTTTGTCGGTACACGATATTCTTCACG-3’ 5’-CGTAATGAGATTTCAGTAAATACAACA-3’ | 55 | Sa442 protein | 108 | [7] |
mecA | 5’-ACCAGATTACAACTTCACCAGG-3’ 5’-CCACTTCATATCTTGTAACG-3’ | 45 | Penicillin binding protein 2a | 162 | [7] |
icaA | 5’-ACACTTGCTGGCGCAGTCAA-3’ 5’-TCTGGAACCAACATCCAACA-3’ | 55 | Intercellular adhesion A | 188 | [24] |
icaD | 5’-ATGGTCAAGCCCAGACAGAG-3’ 5’-AGTATTTTCAATGTTTAAAGCAA-3’ | 55 | Intercellular adhesion D | 198 | [24] |
Can | 5’-GTCAAGCAGTTATTAACACCAGAC-3’ 5’-AATCAGTAATTGCACTTTGTCCACTG-3’ | 62 | Collagen adhesion | 192 | [36] |
fnbA | 5’-CATAAATTGGGAGCAGCATCA-3’ 5’-ATCAGCAGCTGAATTCCCATT-3’ | 62 | Fibronectin binding protein A | 191 | [36] |
fnbB | 5’-GTAACAGCTAATGGTCGAATTGATACT-3’ 5’-CAAGTTCGATAGGAGTACTATGTTC-3’ | 62 | Fibronectin binding protein B | 201 | [36] |
sspA | 5’-GACAACAGCGACACTTGT 3’ 3’-AGTATCTTTACCTACAACTACA-5’ | 45 | Serine protease | 292 | [40] |
sspB | 5’-TGAAGAAGATGGCAAAGTTAG-3’ 3’-TTGAGATACACTTTGTGCAAG-5’ | 47 | Cysteine protease | 493 | [40] |
Geh | 5’-GCACAAGCCTCGG -3’ 3’-GACGGGGGTGTAG-5’ | 40 | Lipase | 473 | [45] |