Fig. 2

Dihydrorhodamine-1,2,3 (DHR) analysis of reactive oxygen species generation by peripheral blood neutrophils. Patient and healthy control cells were incubated with DHR (375 ng/ml), with or without PMA (100 ng/ml), and ROS generation was assayed by FACS analysis. The mean fluorescent intensity (MFI) of the following groups are indicated in the figure: Red lines represent cells from unstimulated healthy controls and patient cells, blue line represents PMA-stimulated patient cells, and grey line represents and PMA-stimulated healthy control cells