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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Quality control implementation for universal characterization of DNA and RNA viruses in clinical respiratory samples using single metagenomic next-generation sequencing workflow

Fig. 4

Evaluation of the metagenomic NGS workflow according to the viral genome type. a Correlation between the results of metagenomic NGS and viral real-time PCR for validated respiratory samples tested positive with viral PCR. Normalized number of reads (RPKM) obtained for targeted virus are displayed against the real-time PCR Ct values for fragmented ssRNA virus (influenza virus) linear dsDNA virus (adenovirus, Epstein-Baar virus, cytomegalovirus, human herpes virus-6) linear ssDNA (human bocavirus) and linear ssRNA (human rhinovirus, respiratory syncytial virus, parainfluenza virus, human coronavirus, human metapneumovirus and measles virus). The correlation coefficients are shown for each viral genome type. b RPKM normalized by Ct for each viral genome type of validated respiratory samples tested positive with viral PCR. Bars show median and interquartile ranges, p-values calculated with the Mann-Whitney U test are shown

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BMC Infectious Diseases

ISSN: 1471-2334

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