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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Quality control implementation for universal characterization of DNA and RNA viruses in clinical respiratory samples using single metagenomic next-generation sequencing workflow

Fig. 2

Determination of optimal spiking level for internal quality control. The sensitivity of the metagenomic analysis workflow for MS2 bacteriophage (Internal Quality Control, IQC) detection was evaluated with a MS2 ten-fold serial dilutions in a nasopharyngeal swab tested negative with multiplex viral PCR. Relative abundance of MS2 bacteriophage and viral families are represented depending on the MS2 spiked-in concentration. IQCT1 corresponds to MS2 molecular detection with commercial real-time PCR assay after amplification step. IQCT2 corresponds to control by sequencing metrics (number of MS2 reads normalized with RPKM ratio and MS2 genome coverage)

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