Fig. 2
Western blot analysis show all eight rabbit antisera generated in this study specifically detected the corresponding Leishmania proteins. The lysate of 4 × 107 Leishmania cells or 1.5 × 105 Human H1299 cells was loaded into each lane and separated on SDS-PAGE. The specific Leishmania proteins were detected with rabbit antisera in 1:1000 dilution and goat anti-rabbit IgG labeled with horseradish peroxidase. Lm pro: L. major promastigotes; Ld pro: L. donovani promastigotes; Ld ama: L. donovani axenic amastigotes; H1299: Human H1299 cell line. Note: similar amounts of these abundant Leishmania proteins were detected in L. major, L. donovani promastigotes, and L. donovani axenic amastigotes, while these sera exhibited variable cross reactivities to human proteins in the H1299 cell lysate