Identification of nine cryptic species of Candida albicans, C. glabrata, and C. parapsilosis complexes using one-step multiplex PCR

Table 3 Expected and actual PCR products on gel are shown. Despite of deviation of amplicon sizes in both individual PCR products and home-made ladder containing mixture of PCR products, target species were clearly distinguishable from each other

Target species	Expecetd PCR product size	Actual home-made ladder PCR product sizes on gel (approximately)	Actual indidividual PCR product size on gel (approximately)
Candida albicans	1090 bps	950 bps	1200 bps
Candida africana	901 bps	800 bps	1100 bps
Candida dubliniensis	721 bps	600 bps	950 bps
Candida nivariensis	588 bps	500 bps	700 bps
Candida orthopsilosis	476 bps	> 400 bps	576 bps
Candida glabrata	386 bps	> 350 bps	486 bps
Candida metapsilosis	308 bps	˜280 bps	400 bps
Candida bracarensis	214 bps	˜200 bps	300 bps
Candida parapsilosis	112 bps	100 bps	250 bps

ISSN: 1471-2334