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Table 3 Detection of H7N9 virus in the clinical samples

From: Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance

Clinical samples

H7 (HP/LP)a

N9 (292R/K)

GISAID NO.

Multiplex qRT-PCR

Kit-1

Kit-2

Sanger sequencing

Multiplex qRT-PCR

Sanger sequencing

SZ01

32.5 (LP)

32.46 (LP)

33.28 (LP)

LP

R

R

EPI_ISL_250424

SZ02

35.92 (LP)

35.85 (LP)

35.52 (LP)

LP

R

R

EPI_ISL_250425

SZ03

31.79 (LP)

31.26 (LP)

31.9 (LP)

LP

R

R

EPI_ISL_250313

SZ04

26.74 (LP)

27.16 (LP)

27.05 (LP)

LP

K

K

EPI_ISL_250314

SZ05

26.04 (HP)

26.74 (HP)

25.28 (HP)

HP

R

R

EPI_ISL_250312

SZ06

26.91 (LP)

26.35 (LP)

26.43 (LP)

LP

R

R

EPI_ISL_250315

SZ07

28.57 (HP)

27.95 (HP)

28.91 (HP)

HP

K

K

EPI_ISL_259757

SZ08

19.81 (HP)

20.29 (HP)

20.22 (HP)

HP

K

K

EPI_ISL_266936

YN01

27.35 (LP)

28.31 (LP)

29.05 (LP)

LP

R

R

EPI_ISL_250316

YN02

36.03 (LP)

35.22 (LP)

35.13 (LP)

LP

R

R

EPI_ISL_250317

  1. aThe Ct values of the commercial kits and our developed method for universal H7 were shown