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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species

Fig. 2

Discriminatory property of the CanTub-simplex PCR. Species level identification was achieved by using CanTub-simplex PCR primer pair to accurately identify Candida species based on HRM analysis (P1) by using the LightCycler®96 HRM analysis Software (Roche Diagnostics, Risch-Rotkreuz, Switzerland). a Representation of the normalized CanTub-simplex PCR melting curves. Each colour represents a different species: Candida albicans (blue) ID1–7; Candida glabrata (red) ID8–11; Candida parapsilosis (yellow) ID12–17; Candida tropicalis (orange) ID18–24; Candida krusei (green) ID25–30; Candida guilliermondii (brown) ID31–34; Candida dubliniensis (pink) ID35–38. b Representation of the normalized difference plots. Normalized difference plots against Candida albicans

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