Table 2 Primer Sequence for nested PCR amplification
From: Coinfections identified from metagenomic analysis of cervical lymph nodes from tularemia patients
Pathogen Target | Nested PCR scheme | Primer Sequence | Amplicon size | Sanger Sequence target | Gene Target | Published |
|---|---|---|---|---|---|---|
Hepatitis B_F1 | Outer Forward | GGGAGGAGATTAGGTTAA | 216 bp | NA | DistalX/pre-C gene | Chakravarty et al., 2002 |
Hepatitis B_R1 | Outer Reverse | GGCAAAAAAGAGAGTAACTC | ||||
Hepatitis B_F1 | Internal Forward | *agctttccttgtttcgaattttataaTCTGTTCACCAGCACCAT | 74 bp | 37 bases | ||
Hepatitis B_R1 | Internal Reverse | AGGCTTGAACAGTAGGACA | ||||
HpB19_F1 | Outer Forward | CAAAAGCATGTGGAGTGAGG | 398 bp | NA | VP1 | Koch and Adler et al., 1990 |
HpB19_R1 | Outer Reverse | CTACTAACATGCATAGGCGC | ||||
HpB19_F1 | Internal Forward | CCCAGAGCACCATTATAAGG | 288 bp | 251 bases | Yamakawa et al., 1995 | |
HpB19_R1 | Internal Reverse | GTGCTGTCAGTAACCTG |