Faecal colonization of E. coli and Klebsiella spp. producing extended-spectrum beta-lactamases and plasmid-mediated AmpC in Mozambican university students

Table 1 Primers used for amplification of ESBL genes

Target enzyme	Primers	Sequence (5′ to 3′)	Annealing temperature (ref)
TEM-1	TEMMF	AAA ATT CTT GAA GAC G	60 °C [29]
TEM-1	TEMMR	TTA CCA ATG CTT AAT CA	60 °C [29]
SHV	SHVMF	TTA ACT CCC TGT TAG CCA	56 °C [29]
SHV	SHVMR	GAT TTG CTG ATT TCG CCC	56 °C [29]
CTX-1	CTXMF	GGT TAA AAA ATC ACT GCG TC	57 °C [27]
CTX-1	CTXMR	TTG GTG ACG ATT TTA GCC GC	57 °C [27]
CMY	CMYMF	GAT TCC TTG GAC TCT TCA G	57 °C [28]
CMY	CMYMR	TAA AAC CAG GTT CCC AGA TAG C	57 °C [28]
FOX	FOXMF	CAC CAC GAG AAT AAC CAT	57 °C [28]
FOX	FOXMR	ATG TGG ACG CCT TGA ACT	57 °C [28]
DHA	DHAMF	AAC TTT CAC AGG TGT GCT GGG T	57 °C [28]
DHA	DHAMR	CCG TAC GCA TAC TGG CTT TGC	57 °C [28]
MOX	MOXMF	GCT GCT CAA GGA GCA CAG GAT	50 °C [28]
MOX	MOXMR	CAC ATT GAC ATA GGT GTG GTG C	50 °C [28]

ISSN: 1471-2334