Fig. 4From: Live attenuated Salmonella typhimurium vaccines delivering SaEsxA and SaEsxB via type III secretion system confer protection against Staphylococcus aureus infectionEvaluation of cellular immune response against rSaEsxA and rSaEsxB by ELISPOT. Mice were immunized with PBS, N106, N19, and N20 strains (n = 3–4/group). Eight or nine days after the second booster, splenocytes were prepared and incubated with specific antigens for 20 h. IFN-γ-producing cells with stimulator rSaEsxA (a) and rSaEsxB (c) were detected by IFN-γ ELISPOT assay. And for IL-17A-producing cells stimulated with rSaEsxA (b) and rSaEsxB (d), IL-17A ELISPOT assays were conducted. SFC, Spot-Forming Cells. Results of one of two experiments are shown. For statistical analysis, student’s t test was used. Data were represented as means ± SEMBack to article page