Fig. 2

Translocation of SaEsxA and SaEsxB fusion proteins into macrophages. Raw264.7 macrophages were infected with ML21, isogenic ΔinvA mutant and ΔsipB mutant, carrying plasmids encoding SipA-SaEsxA (a) and SipA-SaEsxB (b) for 3 h. After lysis with 0.1% Triton X-100, infected macrophages were separated into Triton X 100-insoluble fraction containing intracellular bacteria and Triton X 100-soluble fraction containing bacteria-free cell cytosol. α-tubulin was used as an internal control for protein loading