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Table 1 Detection of anti-DENV IgM by two commercial ELISA tests at initial survey, 6 and 12 months

From: Prolonged persistence of IgM against dengue virus detected by commonly used commercial assays

 

No. DENV IgM positive/total (%)a

Initial

6 months later

12 months later

 

SDb

Focusc

SDb

Focusc

SDb

Focusc

All

44/44 (100.0)

40/44 (90.9)

31/44 (70.5)

6/44 (13.6)

18/39 (46.2)

3/39 (7.7)

p-valued

 

< 0.0001

< 0.0001

Age

 20–50

12/12 (100.0)

8/12 (66.7)

9/12 (75.0)

2/12 (16.7)

5/10 (50.0)

1/10 (10.0)

 51–60

16/16 (100.0)

16/16 (100.0)

12/16 (75.0)

2/16 (12.5)

7/16 (43.8)

1/16 (6.3)

  > 60

16/16 (100.0)

16/16 (100.0)

10/16 (62.5)

2/16 (12.5)

6/13 (46.2)

1/13 (7.7)

 p-valued

0.7676

> 0.9999

> 0.9999

> 0.9999

Sex

 Male

7/7 (100.0)

7/7 (100.0)

6/7 (85.7)

1/7 (14.3)

2/5 (40.0)

1/5 (20.0)

 Female

37/37 (100.0)

33/37 (89.2)

25/37 (67.6)

5/37 (13.5)

16/34 (47.1)

2/34 (5.9)

 p-valued

0.6542

> 0.9999

> 0.9999

0.3452

Initial IgM levele

  > Median

22/22 (100.0)

22/22 (100.0)

19/22 (86.4)

5/22 (22.7)

12/19 (63.2)

3/19 (15.8)

  < Median

22/22 (100.0)

18/22 (81.8)

12/22 (54.5)

1/22 (4.5)

6/20 (30.0)

0/20 (0.0)

 p-value§

0.0452

0.1853

0.0562

0.1060

Diagnosed cases

 Yes

17/17 (100.0)

16/17 (94.1)

11/17 (64.7)

1/17 (5.9)

7/15 (46.7)

1/15 (6.7)

 No

27/27 (100.0)

24/27 (88.9)

20/27 (74.1)

5/27 (18.5)

11/24 (45.8)

2/24 (8.3)

 p-valued

0.5205

0.3801

> 0.9999

> 0.9999

  1. aNumber of positive anti-DENV IgM/number of specimens tested (percentage)
  2. bELISA kits manufactured by Standard Diagnostics
  3. cELISA kits manufactured by Focus Diagnostics
  4. dThe difference in the detection rate among different groups were examined using Fisher’s exact tests
  5. eThe initial anti-DENV IgM level was defined by subtracting the mean the OD values of the negative controls in each plate from the mean OD values of the samples using the SD ELISA