Table 1 Direct laboratory costs at our Institution for standard tissue culture, sonication and MicroDTTect
Direct costs (N = 20 treated patients)/sample | Tissue Culture (5 samples) | Tissue Culture (5 samples) + Sonication | MicroDTTect (pooled 5 samples) | |||||
|---|---|---|---|---|---|---|---|---|
Materials | €/hour | Activities / Product | Minutes | € | Minutes | € | Minutes | € |
Container | 1 | 2 | 350 | |||||
Tubes | 1 | 1 | ||||||
Plates and broths | 5 | 5 | 5 | |||||
Card ID and antibiogram | 10 | 10 | 10 | |||||
Loops, pipettes and others | 2 | 2 | ||||||
Sonicator depreciation and maintenance | 10 | |||||||
Total | 19 | 30 | 365 | |||||
Technician | 25.0 | Pre-analytical phase | 4 | 1.7 | 4 | 1.7 | 2 | 0.8 |
Sample preparation/processing | 27 | 11.3 | 10 | 4.2 | ||||
Sonication | 18 | 7.5 | ||||||
Centrifugation | 15 | 6.3 | 30 | 12.5 | 15 | 6.3 | ||
Seeding | 5 | 2.1 | 30 | 12.5 | 5 | 2.1 | ||
Microscope slide preparation | 10 | 4.2 | 10 | 4.2 | 10 | 4.2 | ||
Vitek loading | 10 | 4.2 | 10 | 4.2 | 10 | 4.2 | ||
Total | 71 | 29.7 | 112 | 46.8 | 42 | 17.6 | ||
Biologist | 40.0 | Pre-analytical phase | 4 | 2.8 | 4 | 2.8 | 1 | 0.7 |
Plates reading | 3 | 2 | 3 | 2 | 3 | 2 | ||
Broth control for 15 days | 10 | 6.7 | 10 | 6.7 | 10 | 6.7 | ||
Control and final validation | 2 | 1.3 | 2 | 1.3 | 2 | 1.3 | ||
Total | 19 | 12.8 | 19 | 12.8 | 16 | 10.7 | ||
Total per Sample | 90 | 61.5 | 131 | 89.6 | 58 | 393.3 | ||
Total per Patient (5 Samples) | 307.5 | 397.1 | 393.3 | |||||