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Table 1 Direct laboratory costs at our Institution for standard tissue culture, sonication and MicroDTTect

From: Cost-benefit analysis of antibiofilm microbiological techniques for peri-prosthetic joint infection diagnosis

Direct costs (N = 20 treated patients)/sample Tissue Culture
(5 samples)
Tissue Culture
(5 samples) + Sonication
MicroDTTect
(pooled 5 samples)
Materials €/hour Activities / Product Minutes Minutes Minutes
   Container   1   2   350
Tubes   1   1   
Plates and broths   5   5   5
Card ID and antibiogram   10   10   10
Loops, pipettes and others   2   2   
Sonicator depreciation and maintenance     10   
Total   19   30   365
Technician 25.0 Pre-analytical phase 4 1.7 4 1.7 2 0.8
Sample preparation/processing 27 11.3 10 4.2   
Sonication    18 7.5   
Centrifugation 15 6.3 30 12.5 15 6.3
Seeding 5 2.1 30 12.5 5 2.1
Microscope slide preparation 10 4.2 10 4.2 10 4.2
Vitek loading 10 4.2 10 4.2 10 4.2
Total 71 29.7 112 46.8 42 17.6
Biologist 40.0 Pre-analytical phase 4 2.8 4 2.8 1 0.7
Plates reading 3 2 3 2 3 2
Broth control for 15 days 10 6.7 10 6.7 10 6.7
Control and final validation 2 1.3 2 1.3 2 1.3
Total 19 12.8 19 12.8 16 10.7
Total per Sample 90 61.5 131 89.6 58 393.3
Total per Patient (5 Samples)   307.5   397.1   393.3