Fig. 1From: An evaluation of a recombinant multiepitope based antigen for detection of Toxoplasma gondii specific antibodiesConstruction of the USM.TOXO1 synthetic gene by assembly PCR. Lane M: 100Â bp DNA Marker. Lane 1: 1st PCR product. Lane 2: Expected USM.TOXO1 gene (456Â bp) amplified in the 2nd PCR. Lane N: Negative controlBack to article page