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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: ORFeome-based identification of biomarkers for serodiagnosis of Mycobacterium tuberculosis latent infection

Fig. 1

Screening of the GST-TBs with serum samples from latent tuberculosis infected (LTBI) individuals. a Serum from 10 LTBI individuals and three healthy controls was used for initial screening. Of 409 GST-TBs, 63 had seropositive reactions, defining the immuno-ORFeome of LTBI. Within the immuno-ORFeome, the rarely hit targets were predominantly latency-associated proteins and secreted proteins (b), while the frequently recognized antigens tended to be membrane-associated proteins (c). Five of eight highly-reactive proteins, Rv0229c, Rv1146, Rv3090, Rv3206c and Rv3921c, were membrane-associated proteins. The ratio of the RLUs was calculated using the formula: R = (RLUs of GST-TB − RLUs of PBS)/(RLUs of GST − RLUs of PBS). GST-TBs with R ≥ 2 were considered to indicate seropositive reactions. Red dots indicated seropositive reactions with R ≥ 2 and black dots indicated seronegative reactions with R< 2. The bars indicated the mean value of each individual antigen. RLUs = relative light units,RD = region of difference

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