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Table 6 Detection rates per assay according to the specimen’s viral titer and sample type

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Sample type Viral titer (PFU/ml) RT-iiPCR No. positive/No. tested CDC-PAHO RT-qPCR No. positive/No. testeda
Whole blood 1 4/20 0/20 (2)
10 11/20 3/20 (6)
102 19/20 17/20
103 20/20 20/20
106 20/20 20/20
Plasma 1 9/20 1/20 (5)
10 18/20 14/20
102 19/20 19/20
103 20/20 20/20
106 20/20 20/20
Serum 1 10/20 1/20 (7)
10 20/20 17/20 (2)
102 20/20 20/20
103 20/20 20/20
106 20/20 20/20
Semen 1 1/4 0/4
10 4/4 3/4
102 4/4 4/4
103 4/4 4/4
106 4/4 4/4
Urine 1 1/20 0/20
10 12/20 1/20 (5)
102 20/20 16/20 (3)
103 20/20 20/20
106 20/20 20/20
Mosquito pools 1 0/1 0/1
10 1/1 1/1
102 1/1 1/1
103 1/1 1/1
104 1/1 1/1
106 1/1 1/1
  1. aNumber between parentheses indicates the number of inconclusive results (Ct > 38.5)
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