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Table 6 Detection rates per assay according to the specimen’s viral titer and sample type

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Sample type

Viral titer (PFU/ml)

RT-iiPCR No. positive/No. tested

CDC-PAHO RT-qPCR No. positive/No. testeda

Whole blood

1

4/20

0/20 (2)

10

11/20

3/20 (6)

102

19/20

17/20

103

20/20

20/20

106

20/20

20/20

Plasma

1

9/20

1/20 (5)

10

18/20

14/20

102

19/20

19/20

103

20/20

20/20

106

20/20

20/20

Serum

1

10/20

1/20 (7)

10

20/20

17/20 (2)

102

20/20

20/20

103

20/20

20/20

106

20/20

20/20

Semen

1

1/4

0/4

10

4/4

3/4

102

4/4

4/4

103

4/4

4/4

106

4/4

4/4

Urine

1

1/20

0/20

10

12/20

1/20 (5)

102

20/20

16/20 (3)

103

20/20

20/20

106

20/20

20/20

Mosquito pools

1

0/1

0/1

10

1/1

1/1

102

1/1

1/1

103

1/1

1/1

104

1/1

1/1

106

1/1

1/1

  1. aNumber between parentheses indicates the number of inconclusive results (Ct > 38.5)