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Table 5 Inclusivity and exclusivity test panel used to compare the specificity of ZIKV RT-qPCR and RT-iiPCR assays

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Virus Lineage ZIKV prM RT-qPCR (Ct value) ZIKV E RT-qPCR (Ct value) ZIKV NS2B RT-qPCR (Ct value) ZIKV RT-iiPCR
ZIKV MR 766 African Neg 18.85 Neg Pos
ZIKV IB H 30656 African 22.58 21.85 Neg Pos
ZIKV PRVABC59 Asian 16.94 17.27 17.81 Pos
ZIKV FLR Asian 18.89 18.97 20.94 Pos
ZIKV H/PAN/2015/CDC-259359 Asian 22.52 23.47 22.75 Pos
ZIKV H/PAN/2015/CDC-259249 Asian 26.85 27.24 26.27 Pos
ZIKV H/PAN/2015/CDC-259364 Asian 26.03 26.46 25.62 Pos
DENV serotype 1, Hawaii N/A Neg Neg Neg Neg
DENV serotype 2, New Guinea C N/A Neg Neg Neg Neg
DENV serotype 3, Philippines/H87/1956 N/A Neg Neg Neg Neg
DENV serotype 4, H241 N/A Neg Neg Neg Neg
YFV 17D N/A Neg Neg Neg Neg
WNV NY99 N/A Neg Neg Neg Neg
CHIKV H20235/STMARTIN/2013 N/A Neg Neg Neg Neg
  1. Pos positive, Neg negative, N/A not applicable
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BMC Infectious Diseases

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