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Table 5 Inclusivity and exclusivity test panel used to compare the specificity of ZIKV RT-qPCR and RT-iiPCR assays
| Virus | Lineage | ZIKV prM RT-qPCR (Ct value) | ZIKV E RT-qPCR (Ct value) | ZIKV NS2B RT-qPCR (Ct value) | ZIKV RT-iiPCR |
|---|---|---|---|---|---|
| ZIKV MR 766 | African | Neg | 18.85 | Neg | Pos |
| ZIKV IB H 30656 | African | 22.58 | 21.85 | Neg | Pos |
| ZIKV PRVABC59 | Asian | 16.94 | 17.27 | 17.81 | Pos |
| ZIKV FLR | Asian | 18.89 | 18.97 | 20.94 | Pos |
| ZIKV H/PAN/2015/CDC-259359 | Asian | 22.52 | 23.47 | 22.75 | Pos |
| ZIKV H/PAN/2015/CDC-259249 | Asian | 26.85 | 27.24 | 26.27 | Pos |
| ZIKV H/PAN/2015/CDC-259364 | Asian | 26.03 | 26.46 | 25.62 | Pos |
| DENV serotype 1, Hawaii | N/A | Neg | Neg | Neg | Neg |
| DENV serotype 2, New Guinea C | N/A | Neg | Neg | Neg | Neg |
| DENV serotype 3, Philippines/H87/1956 | N/A | Neg | Neg | Neg | Neg |
| DENV serotype 4, H241 | N/A | Neg | Neg | Neg | Neg |
| YFV 17D | N/A | Neg | Neg | Neg | Neg |
| WNV NY99 | N/A | Neg | Neg | Neg | Neg |
| CHIKV H20235/STMARTIN/2013 | N/A | Neg | Neg | Neg | Neg |
