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Table 4 Analytical sensitivity of ZIKV RT-qPCR and RT-iiPCR assays using RNA derived from infectious tissue culture fluid (100–10−13)

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

ZIKV PRVABC59a

Dilutions

ZIKV prM RT-qPCR (Ct value)

ZIKV E RT-qPCR (Ct value)

ZIKV NS2B RT-qPCR (Ct value)

ZIKV RT-iiPCR

100

16.92

16.96

16.89

17.22

17.22

17.32

16.61

16.57

16.45

ND

ND

ND

10−1

19.34

19.42

19.36

19.41

19.34

19.43

18.87

18.90

18.86

ND

ND

ND

10−2

23.08

23.06

22.91

23.17

23.21

23.14

22.68

22.65

22.53

ND

ND

ND

10−3

26.02

26.08

26.05

26.18

26.18

26.12

25.50

25.54

25.52

ND

ND

ND

10−4

29.62

29.53

29.63

29.76

29.73

29.9

29.06

29.64

29.72

ND

ND

ND

10−5

32.66

32.50

32.73

33.13

32.94

32.90

32.29

32.01

31.99

Pos

Pos

Pos

10−6

Neg

Neg

Neg

36.89

36.61

36.26

36.94

35.98

36.71

Pos

Pos

Pos

10−7

Neg

Neg

Neg

40.64

Neg

Neg

Neg

Neg

Neg

Pos

Neg

Pos

10−8

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

10−9

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

10−10

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

10−11

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

ND

ND

ND

10−12

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

ND

ND

ND

10−13

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Neg

ND

ND

ND

  1. Detection endpoints (100%) are indicated in bold, and are equivalent to 100 PFU/ml for ZIKV prM RT-qPCR assay, and 10 PFU/ml for ZIKV E and NS2B RT-qPCR assays and ZIKV RT-iiPCR assay. Neg negative, Pos positive, ND not determined; PRVABC59, Puerto Rico 2015 strain (ATCC® VR-1843™); a1x107 plaque forming units per ml (PFU/ml); bold and italic, 100% detection end point
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BMC Infectious Diseases

ISSN: 1471-2334

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