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Table 4 Analytical sensitivity of ZIKV RT-qPCR and RT-iiPCR assays using RNA derived from infectious tissue culture fluid (100–10−13)

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

ZIKV PRVABC59a
Dilutions ZIKV prM RT-qPCR (Ct value) ZIKV E RT-qPCR (Ct value) ZIKV NS2B RT-qPCR (Ct value) ZIKV RT-iiPCR
100 16.92 16.96 16.89 17.22 17.22 17.32 16.61 16.57 16.45 ND ND ND
10−1 19.34 19.42 19.36 19.41 19.34 19.43 18.87 18.90 18.86 ND ND ND
10−2 23.08 23.06 22.91 23.17 23.21 23.14 22.68 22.65 22.53 ND ND ND
10−3 26.02 26.08 26.05 26.18 26.18 26.12 25.50 25.54 25.52 ND ND ND
10−4 29.62 29.53 29.63 29.76 29.73 29.9 29.06 29.64 29.72 ND ND ND
10−5 32.66 32.50 32.73 33.13 32.94 32.90 32.29 32.01 31.99 Pos Pos Pos
10−6 Neg Neg Neg 36.89 36.61 36.26 36.94 35.98 36.71 Pos Pos Pos
10−7 Neg Neg Neg 40.64 Neg Neg Neg Neg Neg Pos Neg Pos
10−8 Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg
10−9 Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg
10−10 Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg Neg
10−11 Neg Neg Neg Neg Neg Neg Neg Neg Neg ND ND ND
10−12 Neg Neg Neg Neg Neg Neg Neg Neg Neg ND ND ND
10−13 Neg Neg Neg Neg Neg Neg Neg Neg Neg ND ND ND
  1. Detection endpoints (100%) are indicated in bold, and are equivalent to 100 PFU/ml for ZIKV prM RT-qPCR assay, and 10 PFU/ml for ZIKV E and NS2B RT-qPCR assays and ZIKV RT-iiPCR assay. Neg negative, Pos positive, ND not determined; PRVABC59, Puerto Rico 2015 strain (ATCC® VR-1843™); a1x107 plaque forming units per ml (PFU/ml); bold and italic, 100% detection end point