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Table 3 Analytical sensitivity of ZIKV RT-qPCR and RT-iiPCR assays using ZIKV in vitro transcribed RNA

From: Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

  ZIKV prM RT-qPCR ZIKV E RT-qPCR ZIKV NS2B RT-qPCR ZIKV RT-iiPCR
ZIKV RNA copies/μl No. positive/No. tested Rate (%) No. positive/No. tested Rate (%) No. positive/No. tested Rate (%) No. positive/No. tested Rate (%)
107 6/6 100 6/6 100 6/6 100 6/6 100
106 6/6 100 6/6 100 6/6 100 6/6 100
105 6/6 100 6/6 100 6/6 100 6/6 100
104 6/6 100 6/6 100 6/6 100 6/6 100
103 3/6 50 6/6 100 6/6 100 6/6 100
102 1/6 17 6/6 100 6/6 100 5/6 83
10 0/6 0 5/6 83 6/6 100 5/6 83
1 0/6 0 1/6 17 1/6 17 1/6 17
0.1 0/6 0 0/6 0 0/6 0 0/6 0
  1. PRVABC59, Puerto Rico 2015 strain (ATCC® VR-1843™)
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