Table 1 Primers used for SNPs of FoxP3 gene
From: G allele at −924 A > G position of FoxP3 gene promoter as a risk factor for tuberculosis
Position | Method | Primer Sequences | Allele Phenotypes |
|---|---|---|---|
−3279 A > C (rs3761548) | Forward: CTGGCTCTCTCCCCAACTGA Forward: TGGCTCTCTCCCCAACTGC Common Reverse: ACAGAGCCCATCATCAGACTCTCTA | A: 334 bp C: 333 bp | |
−924 A > G (rs2232365) | PCR-SSP | Forward: CCCAGCTCAAGAGACCCCA Reverse: GGGCTAGTGAGGAGGCTATTGTAAC Forward: CCAGCTCAAGAGACCCCG Reverse: GCTATTGTAACAGTCCTGGCAAGTG | A: 442 bp G: 427 bp |
FoxP3 gene | qRT- PCR | Forward: CAGCTGCCCACACTGCCCCTAG Reverse: CATTTGCCAGCAGTGGGTAG | |
GAPDH gene | Forward: CCAGGTGGTCTCCTCTGACTTCAACA Reverse: AGGGTCTCTCTCTTCCTCTTGTGCTCT |