Investigation of Salmonella Enteritidis outbreaks in South Africa using multi-locus variable-number tandem-repeats analysis, 2013-2015

Table 1 MLVA loci and PCR primer sequences

Target gene locus	PCR primer	Primer sequence (5′ to 3′)	Expected fragment sizes (bp)	VNTR repeat length (bp)	VNTR references
SENTR7	SENTR7-F	6FAM-ACGATCACCACGGTCACTTC	117–135	9	[15]
SENTR7	SENTR7-R	CGGATAACAACAGGACGCTTC	117–135	9	[15]
SENTR5	SENTR5-F	6FAM-CACCGCACAATCAGTGGAAC	235–301	6	[15]
SENTR5	SENTR5-R	GCGTTGAATATCGGCAGCATG	235–301	6	[15]
SENTR6	SENTR6-F	NED-ATGGACGGAGGCGATAGAC	173–236	7	[15]
SENTR6	SENTR6-R	AGCTTCACAATTTGCGTATTCG	173–236	7	[15]
SENTR4	SENTR4-F	VIC-GACCAACACTCTATGAACCAATG	112–147	7	[15]
SENTR4	SENTR4-R	ACCAGGCAACTATTCGCTATC	112–147	7	[15]
SE-3	SE-3-F	VIC-CAACAAAACAACAGCAGCAT	308–320	12	[15]
SE-3	SE-3-R	GGGAAACGGTAATCAGAAAGT	308–320	12	[15]

ISSN: 1471-2334