Assessment of the effect of larval source management and house improvement on malaria transmission when added to standard malaria control strategies in southern Malawi: study protocol for a cluster-randomised controlled trial

Table 3 Outcomes of interest to be analysed

Outcome	Metric	Source of Data
Entomological inoculation rate (EIR)*	abundance of female malaria vectors collected per trap-night, multiplied by proportion^† positive for Plasmodium falciparum sporozoites; indoor-only, outdoor-only, and pooled*	Routine monitoring of adult mosquitoes
Malaria vector community composition	Ratio^† of An. funestus to An. gambiae to An. arabiensis	Routine monitoring of adult mosquitoes
Malaria vector human blood index (HBI)	Proportion^† of Anopheles with human blood in abdomen out of all blood-fed Anopheles	Resting mosquito collections
Peak malaria vector biting time	Time of day (starting hour to ending hour) when 80% of host-seeking malaria vectors collected	Human landing collections
Larval mosquito density	Number of 3rd instar, 4th instar, and pupae per metre of potential larval habitat	Routine monitoring of larval mosquitoes
Parasite prevalence in children aged 6–59 months	Proportion^† of RDT tests positive for Plasmodium falciparum	Malaria indicator surveys
Prevalence of anaemia in children aged 6–59 months	Proportion^† of anemia tests with Hb < 8.0	Malaria indicator surveys
Incidence of clinical malaria in children aged 6–59 months	Number of clinical malaria cases per child per year	Incidence study cohorts

*Primary outcome^†Raw data for proportions will be stored as separate numbers in the database, with actual proportions calculated at time of analysis only; also applies to ratios.

ISSN: 1471-2334