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Table 3 Summary of the results of nested PCR and our LAMP method combined with MinION™ sequencer

From: A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer

  

Sequence confirmation using MinION™ sequencer

P.f.

P.v.

P.f./P.v.

P.o.w.

P.o.c.

P.k.

P.m.

Neg

Nested PCR

P.f. (n = 36)

36

0

0

0

0

0

0

0

P.v. (n = 17)

0

17

0

0

0

0

0

0

P.f./P.v. (n = 1)

0

0

1

0

0

0

0

0

P.o. (n = 0)

0

0

0

0

0

0

0

0

P.k (n = 0)

0

0

0

0

0

0

0

0

P.m. (n = 0)

0

0

0

0

0

0

0

0

Neg (n = 9)

1

0

0

0

0

0

0

8

Total (n = 63)

37

17

1

0

0

0

0

8

  1. P.f., P. falciparum; P.v. P. vivax; P.o., P. ovale; P.o.w., P. ovale wallikeri; P.o.c., P. ovale crutisi; P.k., P. knowlesi; P.m., P. malariae; Neg, negative