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Table 1 Nucleotide sequences of constructed LAMP primers for 18S–rRNA

From: A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer

Primer

Sequence (5′ - 3′)

Length

F3

GAAATTCTAAGATTTTCTGGAGAC

24

B3

AGTTTTCCCGTGTTGAGT

18

FIP

TATGGTTAAGATTACGACGGTATCTG-RCAACTGCGAAGGCATW

43

BIP

TGGGTTCTGGGGCGAGTATT-CAAATTAAGCCGCAAGCT

38

LF

CACTCCCTTAACTTTCGTTCTTGAT

25

LB

CGCGCAAGCGAGAAAGTTA

19

  1. F3 and B3, outer primers; FIP and BIP, inner primers; LF and LB, loop primers
  2. The FIP primer consisted of F2 and the complementary strand of F1 (F1c)
  3. The BIP primer consisted of B2 and the complementary strand of B1 (B1c)
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BMC Infectious Diseases

ISSN: 1471-2334

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