Fig. 3
Intracellular growth control of M. tuberculosis by monocytes from T2D patients and healthy volunteers. A total of 1 × 105 monocytes/well were placed in a 96-well plate and incubated for 1 h at 37 °C, 5% CO2. The monocytes were infected with M. tuberculosis at MOI 10 for 1 h, and extracellular M. tuberculosis was eliminated by washing. After 1 h (D0), 24 h (D1) and 72 h (D3) of incubation, the supernatants were recovered, the infected monocytes were lysed and M. tuberculosis was counted with a CFU assay. a The dot graph depicts the M. tuberculosis intracellular growth in monocytes T2D patients, n = 51, (closed symbols), and Healthy volunteers, n = 38, (open symbols). *p ≤ 0.05, T2D and Healthy, D0 vs. D1and D3; and D1 vs. D3 (Wilcoxon test). b The dot graph depicts the intracellular growth of M. tuberculosis according to the 25OHD3 serum levels T2D patients <50 nM, n = 27, (grey symbols) and ≥50 nM, n = 23, (closed symbols). *p ≤ 0.05, 25OHD3 < 50 nM vs. ≥50 nM at D0 and D3,(Mann-Whitney U test). In all graphics the median is represented by horizontal line