Fig. 2
Phagocytosis of M. tuberculosis by monocytes from T2D patients and healthy volunteers. A total of 1 × 105 monocytes/well were placed in a chamber slide and adhered during a 1-h incubation at 37 °C, 5% CO2. Monocytes were infected with M. tuberculosis at MOI 10.. Phagocytosis was carried out during a 1-h incubation, and then extracellular M. tuberculosis was eliminated by washing and fixing the cells. Monocytes were stained and analysed with microscopy at 100X with immersion oil. In total, 300–400 monocytes were counted, and monocytes with intracellular M. tuberculosis were reported as the phagocytosis percentage. Representative images show the phagocytosis of M. tuberculosis in monocytes from a T2D patients and b Healthy volunteers; (the arrows indicate the monocytes with intracellular M. tuberculosis). c The dot graph shows the phagocytosis by monocytes from T2D patients, n = 26, (closed circles) and Healthy volunteers, n = 26, (open circles). p > 0.05, T2D vs. Healthy (Mann-Whitney U test). The median is the horizontal line