Table 1 Mutations detected by qPCR-HRM and confirmed by DNA sequencing
Rifampicin phenotypic profile | Amino Acid Change | Nucleotide Change | Number of mutations (Percentage) | qPCR-HRM assay | DNA Sequencing |
|---|---|---|---|---|---|
Resistant (45) | S531L | TCG>TTG | 32 (71.1%) | Mutant | Mutant |
S531W | TCG>TGG | 2 (4.4%) | Mutant | Mutant | |
H526Y | CAC>TAC | 2 (4.4%) | Mutant | Mutant | |
H526L | CAC>CTC | 1 (2.2%) | Mutant | Mutant | |
H526C | CAC>TGC | 3 (6.7%) | Mutant | Mutant | |
No mutation | - | 5 (11.1%) | Wild | Wild | |
Susceptible (22) | No mutation | - | -- | Wild | Wild |