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Table 1 Effect of decontamination time on culture results in cetylpyridinium chloride (CPC) treated and non-CPC samples from tuberculosis (TB) patients in Tanzania

From: Preservation of sputum samples with cetylpyridinium chloride (CPC) for tuberculosis cultures and Xpert MTB/RIF in a low-income country

Culture grading (WHO)

Decontamination of CPC treated samples, n (%)

20 min1

15 min2

10 min3

Non-CPC

CPC

Non-CPC

CPC

Non-CPC

CPC

Contaminated

7 (14)

1 (2)

8 (16)

1 (2)

7 (14)

0 (0)

Negative

17 (34)

4 (8)

8 (16)

2 (4)

6 (12)

3 (6)

Positive

26 (52)

45 (90)

34 (64)

47 (94)

37 (74)

47 (94)

 < 20 colonies

2 (4)

3 (6)

2 (4)

2 (4)

0 (0)

0 (0)

 1+

5 (10)

7 (14)

11(22)

9 (18)

4 (8)

7 (14)

 2+

10 (20)

17 (34)

11 (22)

18(36)

12(24)

15 (30)

 3+

9(18)

18 (36)

10 (20)

18 (36)

21(42)

25 (50)

Total

50 (100)

50 (100)

50 (100)

50 (100)

50 (100)

50 (100)

  1. Difference between CPC treated samples and non-CPC according to WHO culture grading (contaminated, negative, positive): p < 0.0011, p = 0.0042, and p = 0.0073, respectively (Fisher’s exact test)
  2. Culture results of 150 sputum samples split into two equal aliquots, one of which was treated with CPC before processing, with varying times of decontamination