Table 2 Polymerase chain reaction primers and amplicons
SNP | Direction | Sequence (5′-3′) | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
rs12998782 | Forward | AGGAGCTGCAGGTGATAGGAA | 118 | 60.62 |
Reverse | GGAGCCCAAGGGAATGTGTG | 60.97 | ||
rs17009726 | Forward | AAACCCACCTGCCCCTATCA | 150 | 60.85 |
Reverse | CACTAGCCTGCACTGACCAC | 60.67 | ||
rs1194182 | Forward | CATTTGGCTCAGGTGTCAGG | 134 | 61.65 |
Reverse | CACAGGCTCTCAACCCTTCAT | 61.59 | ||
rs3211956 | Forward | CACTTGTGCCAAAGTTGTCC | 126 | 59.19 |
Reverse | TACATGCAGCAATCCTGGTC | 59.68 | ||
rs10499859 | Forward | AGTTCTGGGCAAATGTATGTCCT | 185 | 59.99 |
Reverse | TGCTTGGCTGGTTAGTTTCCA | 60.13 |