Comparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with Plasmodium falciparum field isolates

Jean Claude Biteghe Bi Essone; Nadine N’Dilimabaka; Julien Ondzaga; Jean Bernard Lekana-Douki; Dieudonné Nkoghe Mba; Philippe Deloron; Dominique Mazier; Frédrérick Gay; Fousseyni S. Touré Ndouo

doi:10.1186/s12879-017-2552-0

Table 1 Cytoadherence and apoptosis of HLEC and hCMEC/D3 mediated by P. falciparum infected red blood cell

From: Comparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with Plasmodium falciparum field isolates

Pf isolate	Clinical status	Number of pRBC adhered per 700 cells		Mean OD direct contact mediated apoptosis (SD)		Mean OD soluble factor mediated apoptosis (SD)
Pf isolate	Clinical status	hCMEC/D3	HLEC	HLEC	hCMEC/D3	HLEC	hCMEC/ D3
F1	UN	1	641	0.99 (0.05)	9.08 (0.05)	2.23 (0.09)	8.04 (0.61)
F2	UN	1	1	1.57 (0.12)	8.73 (0.62)	0.83 (0.04)	5.41 (0.07)
F3	UN	25	41	0.86 (0.04)	1.12 (0.09)	0.71 (0/09)	0.46 (0.05)
3D7¹	-	254	415	3.15	3.79	1.25	4.52
RBC¹	-	1	0	0.39	0.49	0.25	0.41
F4	UN	3152	201	2.79 (0.07)	1.68 (0.14)	0.74 (0.04)	0.93 (0.05)
F5	UN	806	54	1.79 (0.08)	1.57 (0.11)	0.62 (0.02)	0.63 (0.18)
3D7²	-	279	361	3.65	3.21	1.05	4.02
RBC²	-	3	8	0.67	0.23	0.42	0.29
F15	UN	69	81	6.39 (0.46)	11.37 (0.21)	2.23 (0.07)	8.59 (0.23)
F16	UN	3	9	1.00 (0.04)	0.68 (0.09)	0.41 (0.05)	1.70 (0.11)
F17	UN	9	41	0.82 (0.09)	0.84 (0.04)	0.73 (0.04)	8.73 (0.69)
3D7³	-	291	541	3.19	3.52	1.88	4.45
RBC³	-	0	0	0.95	0.32	0.89	0.48
F19	UN	7	11	0.71 (0.03)	0.69 (0.06)	0.60 (0.05)	9.08 (0.09)
3D7⁴	-	213	245	3.43	3.91	1.25	4.58
RBC⁴	-	10	6	0.20	0.88	0.11	0.61
F20	UN	51	157	2.55 (0.08)	0.92 (0.13)	0.63 (0.04)	0.54 (0.06)
F21	UN	0	1	2.07 (0.09)	0.95 (0.06)	1.56 (0.30)	0.59 (0.08)
F23	UN	5	54	2.87 (0.09)	0.89 (0.01)	1.09 (0.06)	0.74 (0.03)
3D7⁵	-	259	354	3.00	3.25	1.56	4.12
RBC⁵	-	2	2	0.38	0.14	0.46	0.23
F25^a	SM	5040	2340	0.40 (0.12)	0.74 (0.04)	1.20 (0.10)	0.55 (0.04)
F26^b	SM	1	3	2.08 (0.19)	3.09 (0.06)	3.48 (0.03)	1.88 (0.15)
F27	UN	1652	224	0.63 (0.04)	0.32 (0.11)	1.09 (0.11)	0.66 (0.04)
F28	UN	3	5	2.07 (0.09)	0.42 (0.04)	3.53 (0.08)	0.88 (0.16)
3D7⁶	-	368	463	3.65	4.01	1.12	4.85
RBC⁶	-	3	2	0.52	0.26	0.44	0.31
F29	UN	231	87	2.07 (0.10)	1.62 (0.06)	3.40 (0.10)	3.81 (0.11)
F30	UN	473	286	4.12 (0.14)	5.43 (0.26)	1.97 (0.04)	8.68 (0.13)
F31	UN	337	307	0.38 (0.12)	1.30 (0.34)	4.87 (0.10)	6.75 (0.18)
F33	UN	7	31	1.46 (0.09)	4.74 (0.20)	2.49 (0.06)	1.74 (0.05)
3D7⁷	-	363	435	3.02	3.85	1.00	5.24
RBC⁷	-	0	1	0.54	0.45	0.35	0.15
F34	UN	10	67	1.10 (0.07)	2.23 (0.14)	1.84 (0.08)	2.73 (0.05)
F32	UN	70	157	1.05 (0.07)	4.32 (0.17)	1.07 (0.05)	1.91 (0.11)
3D7⁸	-	440	522	3.75	4.12	1.89	5.87
RBC⁸	-	1	1	0.23	0.46	0.23	0.31
F35	UN	180	101	1.78 (0.11)	1.44 (0.10)	1.76 (0.14)	4.47 (0.09)
F36	UN	7	11	0.94 (0.07)	1.67 (0.08)	0.99 (0.01)	5.92 (0.09)
F37	UN	3	2	3.39 (0.30)	3.00 (0.15)	2.23 (0.04)	6.72 (0.10)
3D7⁹	-	304	354	3.15	3.82	1.46	4.54
RBC⁹	-	3	0	0.65	0.24	0.69	0.36
F39	UN	1	21	1.57 (0.12)	1.53 (0.07)	0.44 (0.06)	1.03 (0.05)
F40	UN	70	196	3.79 (0.14)	3.50 (0.05)	0.50 (0.04)	0.99 (0.01)
3D7¹⁰	-	343	398	3.05	3.81	0.96	4.21
RBC¹⁰	-	-	-	0.52	0.26	0.66	0.17

Pf P. falciparum, UN uncomplicated malaria, SM severe malaria. The number of adherent pRBC was determined microscopically; OD optical density. The mean OD was determined for each isolate. Using a positivity cut-off of 3; 3D7¹: positive control for the first experiment. 3D7^2.3.…10: positive control for the remaining experiments; RBC¹: negative control for the first experiment. RBC^2.3.…10: negative control for the remaining experiments
^aPatient with convulsions
^bPatient with prostration

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