Table 1 Cytoadherence and apoptosis of HLEC and hCMEC/D3 mediated by P. falciparum infected red blood cell
Pf isolate | Clinical status | Number of pRBC adhered per 700 cells | Mean OD direct contact mediated apoptosis (SD) | Mean OD soluble factor mediated apoptosis (SD) | |||
|---|---|---|---|---|---|---|---|
hCMEC/D3 | HLEC | HLEC | hCMEC/D3 | HLEC | hCMEC/ D3 | ||
F1 | UN | 1 | 641 | 0.99 (0.05) | 9.08 (0.05) | 2.23 (0.09) | 8.04 (0.61) |
F2 | UN | 1 | 1 | 1.57 (0.12) | 8.73 (0.62) | 0.83 (0.04) | 5.41 (0.07) |
F3 | UN | 25 | 41 | 0.86 (0.04) | 1.12 (0.09) | 0.71 (0/09) | 0.46 (0.05) |
3D71 | - | 254 | 415 | 3.15 | 3.79 | 1.25 | 4.52 |
RBC1 | - | 1 | 0 | 0.39 | 0.49 | 0.25 | 0.41 |
F4 | UN | 3152 | 201 | 2.79 (0.07) | 1.68 (0.14) | 0.74 (0.04) | 0.93 (0.05) |
F5 | UN | 806 | 54 | 1.79 (0.08) | 1.57 (0.11) | 0.62 (0.02) | 0.63 (0.18) |
3D72 | - | 279 | 361 | 3.65 | 3.21 | 1.05 | 4.02 |
RBC2 | - | 3 | 8 | 0.67 | 0.23 | 0.42 | 0.29 |
F15 | UN | 69 | 81 | 6.39 (0.46) | 11.37 (0.21) | 2.23 (0.07) | 8.59 (0.23) |
F16 | UN | 3 | 9 | 1.00 (0.04) | 0.68 (0.09) | 0.41 (0.05) | 1.70 (0.11) |
F17 | UN | 9 | 41 | 0.82 (0.09) | 0.84 (0.04) | 0.73 (0.04) | 8.73 (0.69) |
3D73 | - | 291 | 541 | 3.19 | 3.52 | 1.88 | 4.45 |
RBC3 | - | 0 | 0 | 0.95 | 0.32 | 0.89 | 0.48 |
F19 | UN | 7 | 11 | 0.71 (0.03) | 0.69 (0.06) | 0.60 (0.05) | 9.08 (0.09) |
3D74 | - | 213 | 245 | 3.43 | 3.91 | 1.25 | 4.58 |
RBC4 | - | 10 | 6 | 0.20 | 0.88 | 0.11 | 0.61 |
F20 | UN | 51 | 157 | 2.55 (0.08) | 0.92 (0.13) | 0.63 (0.04) | 0.54 (0.06) |
F21 | UN | 0 | 1 | 2.07 (0.09) | 0.95 (0.06) | 1.56 (0.30) | 0.59 (0.08) |
F23 | UN | 5 | 54 | 2.87 (0.09) | 0.89 (0.01) | 1.09 (0.06) | 0.74 (0.03) |
3D75 | - | 259 | 354 | 3.00 | 3.25 | 1.56 | 4.12 |
RBC5 | - | 2 | 2 | 0.38 | 0.14 | 0.46 | 0.23 |
F25a | SM | 5040 | 2340 | 0.40 (0.12) | 0.74 (0.04) | 1.20 (0.10) | 0.55 (0.04) |
F26b | SM | 1 | 3 | 2.08 (0.19) | 3.09 (0.06) | 3.48 (0.03) | 1.88 (0.15) |
F27 | UN | 1652 | 224 | 0.63 (0.04) | 0.32 (0.11) | 1.09 (0.11) | 0.66 (0.04) |
F28 | UN | 3 | 5 | 2.07 (0.09) | 0.42 (0.04) | 3.53 (0.08) | 0.88 (0.16) |
3D76 | - | 368 | 463 | 3.65 | 4.01 | 1.12 | 4.85 |
RBC6 | - | 3 | 2 | 0.52 | 0.26 | 0.44 | 0.31 |
F29 | UN | 231 | 87 | 2.07 (0.10) | 1.62 (0.06) | 3.40 (0.10) | 3.81 (0.11) |
F30 | UN | 473 | 286 | 4.12 (0.14) | 5.43 (0.26) | 1.97 (0.04) | 8.68 (0.13) |
F31 | UN | 337 | 307 | 0.38 (0.12) | 1.30 (0.34) | 4.87 (0.10) | 6.75 (0.18) |
F33 | UN | 7 | 31 | 1.46 (0.09) | 4.74 (0.20) | 2.49 (0.06) | 1.74 (0.05) |
3D77 | - | 363 | 435 | 3.02 | 3.85 | 1.00 | 5.24 |
RBC7 | - | 0 | 1 | 0.54 | 0.45 | 0.35 | 0.15 |
F34 | UN | 10 | 67 | 1.10 (0.07) | 2.23 (0.14) | 1.84 (0.08) | 2.73 (0.05) |
F32 | UN | 70 | 157 | 1.05 (0.07) | 4.32 (0.17) | 1.07 (0.05) | 1.91 (0.11) |
3D78 | - | 440 | 522 | 3.75 | 4.12 | 1.89 | 5.87 |
RBC8 | - | 1 | 1 | 0.23 | 0.46 | 0.23 | 0.31 |
F35 | UN | 180 | 101 | 1.78 (0.11) | 1.44 (0.10) | 1.76 (0.14) | 4.47 (0.09) |
F36 | UN | 7 | 11 | 0.94 (0.07) | 1.67 (0.08) | 0.99 (0.01) | 5.92 (0.09) |
F37 | UN | 3 | 2 | 3.39 (0.30) | 3.00 (0.15) | 2.23 (0.04) | 6.72 (0.10) |
3D79 | - | 304 | 354 | 3.15 | 3.82 | 1.46 | 4.54 |
RBC9 | - | 3 | 0 | 0.65 | 0.24 | 0.69 | 0.36 |
F39 | UN | 1 | 21 | 1.57 (0.12) | 1.53 (0.07) | 0.44 (0.06) | 1.03 (0.05) |
F40 | UN | 70 | 196 | 3.79 (0.14) | 3.50 (0.05) | 0.50 (0.04) | 0.99 (0.01) |
3D710 | - | 343 | 398 | 3.05 | 3.81 | 0.96 | 4.21 |
RBC10 | - | - | - | 0.52 | 0.26 | 0.66 | 0.17 |