Fig. 1

Microbiologic outcomes in cerebral spinal fluid. Panel a shows the culture of the specimen obtained from SCF the patient on selective medium for Legionella (BCYE) showing growth of white, dry and rough colonies after 7 days of incubation at 37 °C and 5% CO2 atmosphere. Panels b and c show the microscopic examination of the colonies by Gram and Kinyoun modified strain, showing the presence of ramified, not acid-fast, Gram-positive bacilli. The bacterium was identified by molecular methods of RNAr 16S sequencing, being finally identified as Pseudonocardia carboxydivorans in the National Center of Microbiology (ISCIII, Madrid, Spain)