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Table 2 Mutation distribution of rpoB, katG, inhA promoter and gyrA genes in the smear-positive sputum specimens

From: Early detection of multidrug- and pre-extensively drug-resistant tuberculosis from smear-positive sputum by direct sequencing

Drugs Genes Nucleotide change Amino acid change Number(%) Number of phenotypically resistant strains
rifampicin rpoB a G(1546) → T Asp(516) → Tyr 2(1.59) 1
A(1547) → T Asp(516) → Val 1(1.59) 1
A(1547) → T;A(1552) → Gb Asp(516) → Val;
Asn(518) → Asp
1(1.59) 1
AGA(1550–1552)deletionb Frame shift 1(1.59) 1
C(1576) → T His(526) → Tyr 2(2.18) 2
C(1576) → A His(526) → Asn 2(2.18) 1
C(1576) → G His(526) → Asp 1(1.59) 1
A(1577) → T His(526) → Leu 4(6.35) 4
A(1577) → G His(526) → Arg 1(1.59) 1
A(1577) → C His(526) → Pro 1(1.59) 1
C(1592) → T Ser(531) → Leu 44(68.25) 44
T(1598) → C Leu(533) → Pro 4(6.35) 4
C(1542) → T;ATGGAC(1543–1548)deletionb Frame shift 1(1.59) 1
isoniazid katG G(944) → C Ser(315) → Thr 40(95.24) 40
G(944) → A Ser(315) → Asn 2(4.76) 2
inhA promoter T(−8) → C   1(7.69) 1
C(−15) → T   10(76.92) 9
G(−17) → T   1(7.69) 1
C(−34) → Tc   1(7.69) 1
ofloxacin gyrA C(269) → T Ala(90) → Val 5(14.29) 4
C(269) → G Ala(90) → Gly 2(5.72) 1
T(271) → C Ser(91) → Pro 4(11.43) 4
G(280) → T Asp(94) → Tyr 4(11.43) 3
G(280) → A Asp(94) → Asn 5(14.29) 4
A(281) → G Asp(94) → Gly 12(34.29) 12
A(281) → C Asp(94) → Ala 3(8.57) 3
  1. aFor rpoB gene, E coli codon numbering system is used
  2. b, cthese mutations were not found in the TB Drug Resistance Mutation Database (https://tbdreamdb.ki.se/Info/)