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Table 2 Comparison of five viability markers in eggs deposited in liver and colon tissue from mice infected with S. japonicum

From: Clinical diagnostic value of viable Schistosoma japonicum eggs detected in host tissues

Staining method Marker Positive rate (positive/total eggs; %) at different days post infection
Eggs in hepatic tissue Eggs in colon tissue
45d 90d 180d 45d 90d 180d
  Viable morphology 90.7
(3857/4253)
54.2*
(2510/4631)
35.0*
(1511/4318)
91.1
(4251/4666)
53.8*
(2604/4840)
35.5*
(1609/4532)
NBT/
BCIP
ALP 100.00
(368/368)
82.25
(306/372
48.54
(234/482)
100.00
(287/287)
74.36
(302/406)
66.59
(281/422)
von
Kossa
CalS 100.00
(460/460)
63.86*
(440/689)
43.14
(280/649)
100.00
(378/378)
45.89*
(179/390)
35.87*
(155/432)
DAB AOS 92.68
(2192/2365)
54.05*
(1308/2420)
42.70
(889/2082)
93.45
(2170/2322)
46.03*
(976/2120)
36.28*
(755/2086)
MTT SDHG 98.29
(1607/1635)
60.42*
(815/1349)
50.52
(776/1536)
99.19
(1348/1359)
63.71*
(741/1163)
59.36
(631/1063)
  1. ALP (alkaline phosphatase), CalS (calcified substance), AOS (antioxidase) and SDHG (succinic dehydrogenase). : Data are non-calcified number of eggs. *: Compared with ALP, P < 0.05. All the data were collected from examining 18 blocks of liver and intestinal tissue samples from each of six mice