Table 2 Comparison of five viability markers in eggs deposited in liver and colon tissue from mice infected with S. japonicum
From: Clinical diagnostic value of viable Schistosoma japonicum eggs detected in host tissues
Staining method | Marker | Positive rate (positive/total eggs; %) at different days post infection | |||||
|---|---|---|---|---|---|---|---|
Eggs in hepatic tissue | Eggs in colon tissue | ||||||
45d | 90d | 180d | 45d | 90d | 180d | ||
Viable morphology | 90.7 (3857/4253) | 54.2* (2510/4631) | 35.0* (1511/4318) | 91.1 (4251/4666) | 53.8* (2604/4840) | 35.5* (1609/4532) | |
NBT/ BCIP | ALP | 100.00 (368/368) | 82.25 (306/372 | 48.54 (234/482) | 100.00 (287/287) | 74.36 (302/406) | 66.59 (281/422) |
von Kossa | CalSâ–¼ | 100.00 (460/460) | 63.86* (440/689) | 43.14 (280/649) | 100.00 (378/378) | 45.89* (179/390) | 35.87* (155/432) |
DAB | AOS | 92.68 (2192/2365) | 54.05* (1308/2420) | 42.70 (889/2082) | 93.45 (2170/2322) | 46.03* (976/2120) | 36.28* (755/2086) |
MTT | SDHG | 98.29 (1607/1635) | 60.42* (815/1349) | 50.52 (776/1536) | 99.19 (1348/1359) | 63.71* (741/1163) | 59.36 (631/1063) |