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Table 1 Cell Permissiveness of Zika Virus (ZIKV)

From: Determination of the Cell Permissiveness Spectrum, Mode of RNA Replication, and RNA-Protein Interaction of Zika Virus

Host cell infected

ZIKV (MR766)

ZIKV (PRVABC59)

Protein

RNA

Protein

RNA

Human fibroblast cells

 HFF (ATCC® SCRC-1041™)

 MRC-5 (ATCC® CCL-171™)

++++

++++

++++

++++

 BJ (ATCC® CRL-2522™)

+/−

+/−

Human Epithelial cells

 ARPE-19 (ATCC® CRL-2302™)

+

+

+

+

 A549 (ATCC® CCL-185™)

++

++

++

++

 HT1080 (ATCC® CCL-121™)

+/−

+/−

+/−

+/−

 Hep-2 (ATCC® CCL-23™)

 HEK 293T(ATCC® CRL-1573™)

+

+

+

+/−

Human Endothelial cells

 SLK (AIDSRP, cat# 9402)

Human blood cells

 CEM/CD4 T cell (AIDSRP, cat# 117)

+/−

+/−

+/−

+/−

 THP-1 (AIDSRP, cat# 9949)

 PBMC (ATCC® PCS-800-011™)

++

++

++

++

Other Human cells

 U-251MG (SIGMA 09063001)

++++

++++

++++

++++

 Neural Stem cell

+++

+++

+++

+++

 SK-N-SH (ATCC® HTB-11™)

++++

++++

++++

++++

Simian cells

 Vero (ATCC® CCL-81™)

++++

++++

++++

++++

 COS7 (ATCC® CRL-1651™)

++++

++++

++++

++++

Mouse cells

 MEF

 NIH3T3 (ATCC® CRL-1658™)

  1. The cells were infected with ZIKV at an MOI of 1 for 48 h, and fixed to perform immunostaining using anti-ZIKV antibody and RISH (RNA in situ hybridization) using the probe against ZIKV genome. Total cells (DAPI) and positive cells (ZIKV protein and/or RNA replication) were counted to calculate the positive rate (Positive rate = positive cell counted / total cells counted). HFF human foreskin fibroblast, MEF mouse embryo fibroblast. -: less than 3%; +/−: 3–5%; +: 5–10%; ++: 10–30%; +++: 30–70%; More than 70% positive rate defines ++++. AIDSRP AIDS Reagent Program