Skip to main content

Table 2 Primers for DENV and CHIKV amplification and sequencing

From: Imported arboviral infections in Italy, July 2014-October 2015: a National Reference Laboratory report

Primers

Sequence (5’- 3’)

Reference

RT-PCR:

[35]

 DEUL

TGGCTGGTGCACAGACAATGGTT

 DEUR

GCTGTGTCACCCAGAATGGCCAT

Nested PCR:

 DENUL

GATCTCAAGAAGGAGCCATGCA

 DENUR

ATGGAACTTCCCTTCTTGAACCA

RT-PCR:

[36]

 CHIK 10264 F

GGCGCCTACTGCTTCTG

 CHIK 11300R

CGACACGCATAGCACCSC

Nested PCR:

 CHIK 10564 F

CCCTTTGGCGCAGGAAGAC

 CHIK 11081R

GACTTGTACGCGGAATTCGG

Back to article page

BMC Infectious Diseases

ISSN: 1471-2334

Contact us