Fig 2

PCR amplification, multiple sequence alignment and standard curve plot. a PCR amplification of kDNA minicircle using parasite DNA from L. donovani, L. major and L.tropica. Product of 200 bp size was obtained in all cases. b Multiple Sequence Alignment using CLUSTAL OMEGA. The amplified kDNA sequences from L. donovani, L. tropica and L. major were sequenced and percentage identity analyzed using Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/). Percent identity is shown in parenthesis. c Standard curve obtained with serially diluted L. donovani kDNA plasmid. SYBR Green 1 based QPCR was carried out using F3 and B3 primers at each dilution. Graph shows Ct value plotted at different copy number of kDNA