Fig. 4
From: Antiviral activity of PHA767491 against human herpes simplex virus in vitro and in vivo
PHA767491 inhibits HSV-1 induced necroptosis and the expression levels of viral proteins after HSV-1 entry. a Schematic view. L929 cells (b), T98G cells (c), and HeLa cells (d) were infected with HSV-1(MOI = 4) for 1h. The cells were washed with PBS and then cultured in virus free medium containing with DMSO or PHA767491 for an addition 8h. The expression levels of the HSV-1 proteins gB and ICP6 were measured by western blot analysis. e L929 cells were infected with HSV-1 (MOI = 4) for 2h and then washed once with PBS. The cells were then cultured in fresh medium containing DMSO or PHA767491 for an additional 16h. Cell viability was assessed by measuring ATP levels