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Table 2 Primer sequences and restriction endonucleases used for RFLP analysis and fragment sizes for identification of SNPs

From: Screening of TNFα, IL-10 and TLR4 single nucleotide polymorphisms in individuals with asymptomatic and chronic cutaneous leishmaniasis in Colombia: a pilot study

SNP Primers (5’-3’) Restriction endonuclease Fragment sizes (bp)
IL-10-819 C/T F, 5- TCA TTC TAT GTG CTG GAG ATG G -3 Mae III CC: 125 + 84
TT: 209
TC: 209 + 125 + 84
R, 5- TTT GGG GGA AGT GGG TAA GAG T -3
TNF-α-308 G/A F, 5- AGG CAA TAG GTT TTG AGG GCC AT -3 NcoI GG: 96 + 20
AA: 116
GA: 116 + 96 + 20
R, 5- ACA CTC CCC ATC CTC CCT GCT -3
TLR4 A/G
(Asp299Gly)
F, 5- GAT TAG CAT ATC TAG ACT ACT ACC TCC ATG -3 I NcoI AA: 248
GG: 222 + 26
GA: 248 + 222 + 26
R, 5- GAT CAA CTT CTG AAA AAG CAT TCC CAC -3
TLR4 C/T
(Thr399Ile)
F, 5 -GGT TGC TGT TCT CAA AGT GAT TTT GGG AGA A- 3 HinfI CC: 406
TT: 380 + 26
CT: 406 + 380 + 26
R, 5 -ACC TGA AGA CTG GAG AGT GAG TTA AAT GTT- 3