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Table 2 Primer sequences and restriction endonucleases used for RFLP analysis and fragment sizes for identification of SNPs

From: Screening of TNFα, IL-10 and TLR4 single nucleotide polymorphisms in individuals with asymptomatic and chronic cutaneous leishmaniasis in Colombia: a pilot study

SNP

Primers (5’-3’)

Restriction endonuclease

Fragment sizes (bp)

IL-10-819 C/T

F, 5- TCA TTC TAT GTG CTG GAG ATG G -3

Mae III

CC: 125 + 84

TT: 209

TC: 209 + 125 + 84

R, 5- TTT GGG GGA AGT GGG TAA GAG T -3

TNF-α-308 G/A

F, 5- AGG CAA TAG GTT TTG AGG GCC AT -3

NcoI

GG: 96 + 20

AA: 116

GA: 116 + 96 + 20

R, 5- ACA CTC CCC ATC CTC CCT GCT -3

TLR4 A/G

(Asp299Gly)

F, 5- GAT TAG CAT ATC TAG ACT ACT ACC TCC ATG -3

I NcoI

AA: 248

GG: 222 + 26

GA: 248 + 222 + 26

R, 5- GAT CAA CTT CTG AAA AAG CAT TCC CAC -3

TLR4 C/T

(Thr399Ile)

F, 5 -GGT TGC TGT TCT CAA AGT GAT TTT GGG AGA A- 3

HinfI

CC: 406

TT: 380 + 26

CT: 406 + 380 + 26

R, 5 -ACC TGA AGA CTG GAG AGT GAG TTA AAT GTT- 3