SNP | Primers (5’-3’) | Restriction endonuclease | Fragment sizes (bp) |
---|---|---|---|
IL-10-819 C/T | F, 5- TCA TTC TAT GTG CTG GAG ATG G -3 | Mae III | CC: 125 + 84 TT: 209 TC: 209 + 125 + 84 |
R, 5- TTT GGG GGA AGT GGG TAA GAG T -3 | |||
TNF-α-308 G/A | F, 5- AGG CAA TAG GTT TTG AGG GCC AT -3 | NcoI | GG: 96 + 20 AA: 116 GA: 116 + 96 + 20 |
R, 5- ACA CTC CCC ATC CTC CCT GCT -3 | |||
TLR4 A/G (Asp299Gly) | F, 5- GAT TAG CAT ATC TAG ACT ACT ACC TCC ATG -3 | I NcoI | AA: 248 GG: 222 + 26 GA: 248 + 222 + 26 |
R, 5- GAT CAA CTT CTG AAA AAG CAT TCC CAC -3 | |||
TLR4 C/T (Thr399Ile) | F, 5 -GGT TGC TGT TCT CAA AGT GAT TTT GGG AGA A- 3 | HinfI | CC: 406 TT: 380 + 26 CT: 406 + 380 + 26 |
R, 5 -ACC TGA AGA CTG GAG AGT GAG TTA AAT GTT- 3 |