Comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and PCR

Table 2 Bacterial identification based on specimen collection method

Type of specimen collection	CM	HC	PCR	Number of specimens negative by all methods (%)
Vitreous tap	n	Strep gr. D	n	4 (50)
	n	S. agalactiae	S. agalactiae
	S. pneumoniae	n	n
	n	S. coagulase negative	n
	n	E. faecalis	E. faecalis
	S. pneumoniae	S. pneumoniae	n
	n	n	I
PPV	n	n	H. influenzae	6 (31)
	Klebsiella pneumoniae	Klebsiella pneumoniae	Klebsiella pneumoniae
	S. agalactiae	S. agalactiae	S. agalactiae
	n	E. faecalis	E. faecalis
	n	E. faecalis	E. faecalis
	n	n	E. faecalis
	S. coagulase neg	S. coagulase neg	S. coagulase neg
Evisceration	n	Strep gr. D	S. vestibularis	3 (60)
	n	n	H. influenzae
	n	n	I
	n	n	I

CM, conventional method; HC, hemoculture bottle method; PCR, polymerase chain reaction method; n, no growth; I, inconclusive; Vitreous tap, vitreous tapping; PPV, pars plana vitrectomy; Strep gr. D; Streptococcus Group D; S. vestibularis, Streptococcus vestibularis; H. influenzae, Haemophilus influenzae; S. agalactiae, Streptococcus agalactiae; S. pneumoniae, Streptococcus pneumoniae; S coagulase neg, Coagulase-negative staphylococci; E. faecalis, Enterococcus faecalis

ISSN: 1471-2334