Table 3 Performance of RT-SIBA and RT-PCR for the detection of RSV from NP swab specimens using purified RNA or the rapid lysis protocol
From: Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®
Extracted RNA from clinical specimens | Rapid sample processing | |||
|---|---|---|---|---|
Sample no. | Original sample type | RT-SIBA detection time (minutes) | RT-PCR, cycle threshold Ct (result) | RT-SIBA detection time (minutes) |
1 | NP UTM | 10 | 25 (RSV B+) | 16 |
2 | NP UTM | 11 | 27 (RSV B+) | 31 |
3 | NP UTM | 11 | 27 (RSV B+) | 19 |
4 | NP UTM | 10 | 24 (RSV B+) | 22 |
5 | NP UTM | 11 | 26 (RSV B+) | 15 |
6 | NP UTM | 11 | 27 (RSV B+) | 17 |
7 | NP UTM | 11 | 26 (RSV B+) | 15 |
8 | NP UTM | 10 | 23 (RSV B+) | 16 |
9 | NP UTM | 11 | 28 (RSV B+) | 44 |
10 | NP UTM | 12 | 29 (RSV B+) | 20 |
11 | NP UTM | 10 | 23 (RSV B+) | 16 |
12 | NP UTM | 20 | 25 (RSV B+) | 36 |
13 | NP UTM | 14 | 28 (RSV B+) | 35 |
14 | NP UTM | 11 | 28 (RSV B+) | 16 |
15 | NP UTM | 12 | 28 (RSV B+) | 20 |
16 | NP UTM | 10 | 24 (RSV B+) | 19 |
17 | NP UTM | 12 | 26 (RSV A+) | 17 |
18 | NP UTM | 11 | 25 (RSV B+) | 21 |
19 | NP UTM | 11 | 25 (RSV B+) | 16 |
20 | NP UTM | 11 | 33 (RSV B+) | 15 |
21 | NP UTM | 20 | 27 (RSV B+) | 37 |
22 | NP UTM | 10 | 25 (RSV B+) | 15 |
23 | NP VTM | 14 | 31 (RSV A+) | 22 |
24 | NP VTM | 12 | 27 (RSV A+) | 15 |
25 | NP VTM | 13 | 31 (RSV B+) | 21 |
26–40 | NP UTM | ND | RSV (−) | ND |