Table 2 Mean ± standard deviations log10 RF of different liquid antiseptics with and without organic soil
Test compound Type of soil | Exposure time | ||||
|---|---|---|---|---|---|
5 min | 30 min | 3 h | 10 h | 24 h | |
10% PVP-I | |||||
Without soil - S. aureus | 5.4 ± 0.2 | 5.5 ± 0.1 | 5.7 ± 0.1 | 6.0 ± 0.1 | 4.6 ± 0.5 |
E. faecium | 4.5 ± 0.7 | 5.1 ± 0.1 | 5.1 ± 0.1 | n.g. | n.g. |
P. aeruginosa | 5.8 ± 1.3 | 6.3 ± 0.1 | 6.1 ± 0.1 | 6.2 ± 0.1 | n.g. |
MEM + FBS – S. aureus | 4.6 ± 0.5 | 5.1 ± 0.1 | 5.0 ± 0.1 | 5.4 ± 0.1 | 5.5 ± 0.1 |
E. faecium | 4.1 ± 0.6 | 4.6 ± 0.1 | 4.2 ± 0.1 | n.g. | n.g. |
P. aeruginosa | 4.1 ± 0.4 | 6.0 ± 0.1 | 6.1 ± 0.1 | n.g. | n.g. |
CSL+ 30% blood – S. aureus | 1.8 ± 0.7 | 4.2 ± 0.1 | 5.3 ± 0.1 | 6.2 ± 0.1 | 6.5 ± 0.1 |
E. faecium | 1.3 ± 0.2 | 5.1 ± 0.1 | 5.1 ± 0.1 | n.g. | n.g. |
P. aeruginosa | 2.3 ± 0.4 | 6.0 ± 0.1 | 6.1 ± 0.1 | n.g. | n.g. |
0.05% CHG | |||||
Without soil - S. aureus | 1.6 ± 0.2 | 3.7 ± 0.3 | 5.1 ± 0.8 | 5.8 ± 0.2 | 6.3 ± 0.1 |
E. faecium | 0.2 ± 0.1 | 0.6 ± 0.2 | 1.2 ± 0.2 | 2.1 ± 0.2 | 2.6 ± 0.8 |
P. aeruginosa | 0.1 ± 0.2 | 0.4 ± 0.4 | 2.1 ± 0.1 | 4.0 ± 0.7 | 3.0 ± 0.7 |
MEM + FBS – S. aureus | 2.7 ± 0.4 | 3.0 ± 0.8 | 4.7 ± 0.2 | 5.0 ± 0.3 | 5.3 ± 0.1 |
E. faecium | 0.5 ± 0.2 | 2.5 ± 0.4 | 3.6 ± 0.3 | 4.0 ± 0.1 | 4.8 ± 0.1 |
P. aeruginosa | 0.8 ± 0.4 | 1.0 ± 0.2 | 5.4 ± 0.1 | 6.0 ± 0.1 | 6.6 ± 0.1 |
CSL+ 30% blood – S. aureus | 0.8 ± 0.1 | 1.3 ± 0.5 | 3.7 ± 0.4 | 5.9 ± 0.4 | 6.7 ± 0.2 |
E. faecium | 0.3 ± 0.5 | 1.1 ± 0.3 | 1.8 ± 0.5 | 3.4 ± 0.7 | 4.6 ± 0.6 |
P. aeruginosa | 0.1 ± 0.2 | 1.0 ± 0.3 | 4.7 ± 1.0 | 5.8 ± 0.9 | 7.1 ± 0.1 |
0.1% OCT | |||||
Without soil - S. aureus | 4.4 ± 0.1 | 4.5 ± 0.2 | 4.6 ± 0.2 | 5.2 ± 0.1 | 5.7 ± 0.2 |
E. faecium | 1.5 ± 0.5 | 2.1 ± 0.4 | 4.7 ± 1.0 | 5.6 ± 0.1 | 5.1 ± 0.1 |
P. aeruginosa | 1.0 ± 0.5 | 4.8 ± 0.4 | 4.8 ± 0.4 | 5.1 ± 0.2 | 6.2 ± 0.1 |
MEM + FBS – S. aureus | 3.5 ± 0.5 | 3.4 ± 0.6 | 3.7 ± 0.6 | 4.2 ± 0.2 | 5.4 ± 0.2 |
E. faecium | 2.5 ± 0.5 | 4.4 ± 0.4 | 4.4 ± 0.2 | 4.5 ± 0.3 | 4.3 ± 0.2 |
P. aeruginosa | 1.1 ± 1.0 | 3.8 ± 0.3 | 3.8 ± 0.2 | 4.2 ± 0.1 | 5.7 ± 0.1 |
CSL+ 30% blood – S. aureus | 1.2 ± 0.1 | 2.2 ± 0.1 | 5.5 ± 0.1 | 5.8 ± 0.1 | 5.9 ± 0.1 |
E. faecium | 1.3 ± 0.1 | 2.6 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 0.3 ± 0.1 | 0.8 ± 0.1 | 2.2 ± 0.1 | 6.0 ± 0.1 | 6.7 ± 0.1 |
0.05% OCT | |||||
Without soil - S. aureus | 3.2 ± 1.3 | 4.5 ± 0.2 | 4.6 ± 0.2 | 5.2 ± 0.1 | 5.7 ± 0.2 |
E. faecium | 1.4 ± 0.3 | 2.3 ± 0.3 | 3.4 ± 1.8 | 5.6 ± 0.1 | 5.5 ± 0.1 |
P. aeruginosa | 2.1 ± 1.1 | 4.8 ± 0.4 | 4.8 ± 0.4 | 5.5 ± 0.2 | 6.2 ± 0.1 |
MEM + FBS – S. aureus | 3.5 ± 0.5 | 3.4 ± 0.6 | 3.7 ± 0.6 | 4.2 ± 0.2 | 5.4 ± 0.2 |
E. faecium | 2.4 ± 1.2 | 4.4 ± 0.4 | 4.4 ± 0.2 | 4.5 ± 0.3 | 4.3 ± 0.2 |
P. aeruginosa | 1.5 ± 0.1 | 3.8 ± 0.3 | 3.8 ± 0.2 | 4.2 ± 0.1 | 5.7 ± 0.1 |
CSL+ 30% blood – S. aureus | 1.2 ± 0.1 | 1.4 ± 0.1 | 4.6 ± 0.2 | 5.8 ± 0.1 | 5.9 ± 0.1 |
E. faecium | 1.1 ± 0.1 | 2.3 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 0.2 ± 0.1 | 0.8 ± 0.1 | 1.8 ± 0.1 | 6.0 ± 0.1 | 6.7 ± 0.1 |
0.005% OCT | |||||
Without soil – S. aureus | 0.8 ± 0.9 | 4.5 ± 0.2 | 4.6 ± 0.2 | 5.2 ± 0.1 | 5.7 ± 0.2 |
E. faecium | 0.5 ± 0.3 | 0.7 ± 0.3 | 1.4 ± 0.7 | 1.3 ± 0.3 | 2.9 ± 1.8 |
P. aeruginosa | 0.4 ± 0.1 | 1.2 ± 0.4 | 3.1 ± 1.2 | 4.7 ± 1.1 | 5.8 ± 0.8 |
MEM + FBS – S. aureus | 3.1 ± 0.7 | 3.4 ± 0.6 | 3.7 ± 0.6 | 4.2 ± 0.2 | 5.4 ± 0.2 |
E. faecium | 0.9 ± 0.6 | 1.5 ± 0.5 | 2.5 ± 1.2 | 4.5 ± 0.3 | 4.3 ± 0.2 |
P. aeruginosa | 0.9 ± 0.5 | 2.1 ± 1.1 | 3.8 ± 0.2 | 4.2 ± 0.1 | 5.7 ± 0.1 |
0.1% PHMB | |||||
Without soil – S. aureus | 2.4 ± 0.1 | 4.2 ± 0.3 | 5.4 ± 0.1 | 5.4 ± 0.1 | 6.1 ± 0.1 |
E. faecium | 4.8 ± 0.2 | 5.1 ± 0.1 | 5.1 ± 0.1 | 5.2 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 3.5 ± 0.1 | 5.5. ± 0.1 | 5.7 ± 0.1 | 5.8 ± 0.1 | 6.0 ± 0.1 |
MEM + FBS – S. aureus | 1.3 ± 0.1 | 4.2 ± 0.3 | 5.6 ± 0.1 | 5.7 ± 0.1 | 6.1 ± 0.1 |
E. faecium | 4.7 ± 0.3 | 5.2 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 2.4 ± 0.1 | 5.2 ± 0.2 | 5.4 ± 0.1 | 6.1 ± 0.1 | 6.5 ± 0.1 |
CSL+ 30% blood – S. aureus | 1.1 ± 0.1 | 1.7 ± 0.1 | 4.9 ± 0.4 | 5.8 ± 0.1 | 5.9 ± 0.1 |
E. faecium | 2.1 ± 0.1 | 2.1 ± 0.1 | 4.8 ± 0.3 | 5.4 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 0.2 ± 0.1 | 1.3 ± 0.1 | 5.8 ± 0.1 | 6.1 ± 0.1 | 6.8 ± 0.1 |
0.04% PHMB | |||||
Without soil – S. aureus | 1.3 ± 0.1 | 2.6 ± 0.1 | 5.1 ± 0.5 | 5.4 ± 0.1 | 6.1 ± 0.1 |
E. faecium | 3.8 ± 0.1 | 5.1 ± 0.1 | 5.1 ± 0.1 | 5.2 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 2.1 ± 0.1 | 4.5 ± 0.2 | 5.7 ± 0.1 | 5.8 ± 0.1 | 6.0 ± 0.1 |
MEM + FBS – S. aureus | 0.8 ± 0.1 | 1.2 ± 0.1 | 5.6 ± 0.1 | 5.6 ± 0.1 | 6.1 ± 0.1 |
E. faecium | 3.1 ± 0.1 | 5.1 ± 0.2 | 5.4 ± 0.1 | 5.4 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 1.0 ± 0.1 | 3.4 ± 0.1 | 5.4 ± 0.1 | 6.1 ± 0.1 | 6.5 ± 0.1 |
CSL+ 30% blood – S. aureus | 0.9 ± 0.1 | 1.2 ± 0.1 | 3.2 ± 0.1 | 5.8 ± 0.1 | 5.9 ± 0.1 |
E. faecium | 1.5 ± 0.1 | 1.5 ± 0.1 | 2.7 ± 0.1 | 4.1 ± 0.1 | 5.4 ± 0.1 |
P. aeruginosa | 0.1 ± 0.1 | 0.6 ± 0.1 | 4.1 ± 0.1 | 6.1 ± 0.1 | 6.8 ± 0.1 |
0.02% PHMB | |||||
Without soil – S. aureus | −0.1 ± 0.1 | 0.5 ± 0.1 | 2.8 ± 0.3 | 5.2 ± 0.1 | 5.7 ± 0.2 |
E. faecium | 0.3 ± 0.1 | 0.4 ± 0.2 | 0.4 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 |
P. aeruginosa | 0.4 ± 0.6 | 0.2 ± 0.3 | 1.2 ± 0.8 | 1.5 ± 0.4 | 1.4 ± 0.6 |
MEM + FBS – S. aureus | 0.6 ± 0.4 | 1.7 ± 1.3 | 3.7 ± 0.6 | 4.2 ± 0.2 | 5.4 ± 0.2 |
E. faecium | 0.5 ± 0.2 | 1.1 ± 0.3 | 1.1 ± 0.4 | 1.0 ± 0.9 | 1.6 ± 0.5 |
P. aeruginosa | 0.5 ± 0.8 | 0.9 ± 0.1 | 3.0 ± 1.1 | 3.2 ± 1.8 | 3.8 ± 2.1 |
Controls (test strain: S. aureus) | |||||
Gel | −0.1 ± 0.2 | 0.1 ± 0.4 | 0.3 ± 0.2 | 0.9 ± 0.3 | 1.4 ± 0.3 |
Gel + MEM + FBS | 0.1 ± 0.2 | 0.3 ± 0.3 | 0.3 ± 0.2 | 0.8 ± 0.1 | 1.5 ± 0.6 |
Distilled watera | −0.1 ± 0.2 | −0.1 ± 0.2 | 0.3 ± 0.4 | 1.0 ± 0.4 | 1.7 ± 0.6 |