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Table 2 Mean ± standard deviations log10 RF of different liquid antiseptics with and without organic soil

From: Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride

Test compound
Type of soil
Exposure time
5 min 30 min 3 h 10 h 24 h
10% PVP-I
 Without soil - S. aureus 5.4 ± 0.2 5.5 ± 0.1 5.7 ± 0.1 6.0 ± 0.1 4.6 ± 0.5
E. faecium 4.5 ± 0.7 5.1 ± 0.1 5.1 ± 0.1 n.g. n.g.
P. aeruginosa 5.8 ± 1.3 6.3 ± 0.1 6.1 ± 0.1 6.2 ± 0.1 n.g.
 MEM + FBS – S. aureus 4.6 ± 0.5 5.1 ± 0.1 5.0 ± 0.1 5.4 ± 0.1 5.5 ± 0.1
E. faecium 4.1 ± 0.6 4.6 ± 0.1 4.2 ± 0.1 n.g. n.g.
P. aeruginosa 4.1 ± 0.4 6.0 ± 0.1 6.1 ± 0.1 n.g. n.g.
 CSL+ 30% blood – S. aureus 1.8 ± 0.7 4.2 ± 0.1 5.3 ± 0.1 6.2 ± 0.1 6.5 ± 0.1
E. faecium 1.3 ± 0.2 5.1 ± 0.1 5.1 ± 0.1 n.g. n.g.
P. aeruginosa 2.3 ± 0.4 6.0 ± 0.1 6.1 ± 0.1 n.g. n.g.
0.05% CHG
 Without soil - S. aureus 1.6 ± 0.2 3.7 ± 0.3 5.1 ± 0.8 5.8 ± 0.2 6.3 ± 0.1
E. faecium 0.2 ± 0.1 0.6 ± 0.2 1.2 ± 0.2 2.1 ± 0.2 2.6 ± 0.8
P. aeruginosa 0.1 ± 0.2 0.4 ± 0.4 2.1 ± 0.1 4.0 ± 0.7 3.0 ± 0.7
 MEM + FBS – S. aureus 2.7 ± 0.4 3.0 ± 0.8 4.7 ± 0.2 5.0 ± 0.3 5.3 ± 0.1
E. faecium 0.5 ± 0.2 2.5 ± 0.4 3.6 ± 0.3 4.0 ± 0.1 4.8 ± 0.1
P. aeruginosa 0.8 ± 0.4 1.0 ± 0.2 5.4 ± 0.1 6.0 ± 0.1 6.6 ± 0.1
 CSL+ 30% blood – S. aureus 0.8 ± 0.1 1.3 ± 0.5 3.7 ± 0.4 5.9 ± 0.4 6.7 ± 0.2
E. faecium 0.3 ± 0.5 1.1 ± 0.3 1.8 ± 0.5 3.4 ± 0.7 4.6 ± 0.6
P. aeruginosa 0.1 ± 0.2 1.0 ± 0.3 4.7 ± 1.0 5.8 ± 0.9 7.1 ± 0.1
0.1% OCT
 Without soil - S. aureus 4.4 ± 0.1 4.5 ± 0.2 4.6 ± 0.2 5.2 ± 0.1 5.7 ± 0.2
E. faecium 1.5 ± 0.5 2.1 ± 0.4 4.7 ± 1.0 5.6 ± 0.1 5.1 ± 0.1
P. aeruginosa 1.0 ± 0.5 4.8 ± 0.4 4.8 ± 0.4 5.1 ± 0.2 6.2 ± 0.1
 MEM + FBS – S. aureus 3.5 ± 0.5 3.4 ± 0.6 3.7 ± 0.6 4.2 ± 0.2 5.4 ± 0.2
E. faecium 2.5 ± 0.5 4.4 ± 0.4 4.4 ± 0.2 4.5 ± 0.3 4.3 ± 0.2
P. aeruginosa 1.1 ± 1.0 3.8 ± 0.3 3.8 ± 0.2 4.2 ± 0.1 5.7 ± 0.1
 CSL+ 30% blood – S. aureus 1.2 ± 0.1 2.2 ± 0.1 5.5 ± 0.1 5.8 ± 0.1 5.9 ± 0.1
E. faecium 1.3 ± 0.1 2.6 ± 0.1 5.4 ± 0.1 5.4 ± 0.1 5.4 ± 0.1
P. aeruginosa 0.3 ± 0.1 0.8 ± 0.1 2.2 ± 0.1 6.0 ± 0.1 6.7 ± 0.1
0.05% OCT
 Without soil - S. aureus 3.2 ± 1.3 4.5 ± 0.2 4.6 ± 0.2 5.2 ± 0.1 5.7 ± 0.2
E. faecium 1.4 ± 0.3 2.3 ± 0.3 3.4 ± 1.8 5.6 ± 0.1 5.5 ± 0.1
P. aeruginosa 2.1 ± 1.1 4.8 ± 0.4 4.8 ± 0.4 5.5 ± 0.2 6.2 ± 0.1
 MEM + FBS – S. aureus 3.5 ± 0.5 3.4 ± 0.6 3.7 ± 0.6 4.2 ± 0.2 5.4 ± 0.2
E. faecium 2.4 ± 1.2 4.4 ± 0.4 4.4 ± 0.2 4.5 ± 0.3 4.3 ± 0.2
P. aeruginosa 1.5 ± 0.1 3.8 ± 0.3 3.8 ± 0.2 4.2 ± 0.1 5.7 ± 0.1
 CSL+ 30% blood – S. aureus 1.2 ± 0.1 1.4 ± 0.1 4.6 ± 0.2 5.8 ± 0.1 5.9 ± 0.1
E. faecium 1.1 ± 0.1 2.3 ± 0.1 5.4 ± 0.1 5.4 ± 0.1 5.4 ± 0.1
P. aeruginosa 0.2 ± 0.1 0.8 ± 0.1 1.8 ± 0.1 6.0 ± 0.1 6.7 ± 0.1
0.005% OCT
 Without soil – S. aureus 0.8 ± 0.9 4.5 ± 0.2 4.6 ± 0.2 5.2 ± 0.1 5.7 ± 0.2
E. faecium 0.5 ± 0.3 0.7 ± 0.3 1.4 ± 0.7 1.3 ± 0.3 2.9 ± 1.8
P. aeruginosa 0.4 ± 0.1 1.2 ± 0.4 3.1 ± 1.2 4.7 ± 1.1 5.8 ± 0.8
 MEM + FBS – S. aureus 3.1 ± 0.7 3.4 ± 0.6 3.7 ± 0.6 4.2 ± 0.2 5.4 ± 0.2
E. faecium 0.9 ± 0.6 1.5 ± 0.5 2.5 ± 1.2 4.5 ± 0.3 4.3 ± 0.2
P. aeruginosa 0.9 ± 0.5 2.1 ± 1.1 3.8 ± 0.2 4.2 ± 0.1 5.7 ± 0.1
0.1% PHMB
 Without soil – S. aureus 2.4 ± 0.1 4.2 ± 0.3 5.4 ± 0.1 5.4 ± 0.1 6.1 ± 0.1
E. faecium 4.8 ± 0.2 5.1 ± 0.1 5.1 ± 0.1 5.2 ± 0.1 5.4 ± 0.1
P. aeruginosa 3.5 ± 0.1 5.5. ± 0.1 5.7 ± 0.1 5.8 ± 0.1 6.0 ± 0.1
 MEM + FBS – S. aureus 1.3 ± 0.1 4.2 ± 0.3 5.6 ± 0.1 5.7 ± 0.1 6.1 ± 0.1
E. faecium 4.7 ± 0.3 5.2 ± 0.1 5.4 ± 0.1 5.4 ± 0.1 5.4 ± 0.1
P. aeruginosa 2.4 ± 0.1 5.2 ± 0.2 5.4 ± 0.1 6.1 ± 0.1 6.5 ± 0.1
 CSL+ 30% blood – S. aureus 1.1 ± 0.1 1.7 ± 0.1 4.9 ± 0.4 5.8 ± 0.1 5.9 ± 0.1
E. faecium 2.1 ± 0.1 2.1 ± 0.1 4.8 ± 0.3 5.4 ± 0.1 5.4 ± 0.1
P. aeruginosa 0.2 ± 0.1 1.3 ± 0.1 5.8 ± 0.1 6.1 ± 0.1 6.8 ± 0.1
0.04% PHMB
 Without soil – S. aureus 1.3 ± 0.1 2.6 ± 0.1 5.1 ± 0.5 5.4 ± 0.1 6.1 ± 0.1
E. faecium 3.8 ± 0.1 5.1 ± 0.1 5.1 ± 0.1 5.2 ± 0.1 5.4 ± 0.1
P. aeruginosa 2.1 ± 0.1 4.5 ± 0.2 5.7 ± 0.1 5.8 ± 0.1 6.0 ± 0.1
 MEM + FBS – S. aureus 0.8 ± 0.1 1.2 ± 0.1 5.6 ± 0.1 5.6 ± 0.1 6.1 ± 0.1
E. faecium 3.1 ± 0.1 5.1 ± 0.2 5.4 ± 0.1 5.4 ± 0.1 5.4 ± 0.1
P. aeruginosa 1.0 ± 0.1 3.4 ± 0.1 5.4 ± 0.1 6.1 ± 0.1 6.5 ± 0.1
 CSL+ 30% blood – S. aureus 0.9 ± 0.1 1.2 ± 0.1 3.2 ± 0.1 5.8 ± 0.1 5.9 ± 0.1
E. faecium 1.5 ± 0.1 1.5 ± 0.1 2.7 ± 0.1 4.1 ± 0.1 5.4 ± 0.1
P. aeruginosa 0.1 ± 0.1 0.6 ± 0.1 4.1 ± 0.1 6.1 ± 0.1 6.8 ± 0.1
0.02% PHMB
 Without soil – S. aureus −0.1 ± 0.1 0.5 ± 0.1 2.8 ± 0.3 5.2 ± 0.1 5.7 ± 0.2
E. faecium 0.3 ± 0.1 0.4 ± 0.2 0.4 ± 0.1 0.2 ± 0.1 0.1 ± 0.1
P. aeruginosa 0.4 ± 0.6 0.2 ± 0.3 1.2 ± 0.8 1.5 ± 0.4 1.4 ± 0.6
 MEM + FBS – S. aureus 0.6 ± 0.4 1.7 ± 1.3 3.7 ± 0.6 4.2 ± 0.2 5.4 ± 0.2
E. faecium 0.5 ± 0.2 1.1 ± 0.3 1.1 ± 0.4 1.0 ± 0.9 1.6 ± 0.5
P. aeruginosa 0.5 ± 0.8 0.9 ± 0.1 3.0 ± 1.1 3.2 ± 1.8 3.8 ± 2.1
Controls (test strain: S. aureus)
 Gel −0.1 ± 0.2 0.1 ± 0.4 0.3 ± 0.2 0.9 ± 0.3 1.4 ± 0.3
 Gel + MEM + FBS 0.1 ± 0.2 0.3 ± 0.3 0.3 ± 0.2 0.8 ± 0.1 1.5 ± 0.6
 Distilled watera −0.1 ± 0.2 −0.1 ± 0.2 0.3 ± 0.4 1.0 ± 0.4 1.7 ± 0.6
  1. aControl for aqueous solutions without soil