Fig. 3

HBV titre was evaluated in culture supernatants of HepG2.2.15 cells after treatment with 4, 6 and 8μg/ml of TLR7 agonist (R837) for 72 h. a HBV DNA was isolated from the culture media of R837 treated cells and the load was assessed by absolute real-time PCR using WHO standards. b HBsAg and HBeAg were detected from the culture supernatant of treated cells by ELISA