Vaccination with toxofilin DNA in combination with an alum-monophosphoryl lipid A mixed adjuvant induces significant protective immunity against Toxoplasma gondii

Table 5 Mean cyst burden per mouse brain 4 weeks after challenge with 20 cysts of Toxoplasma gondii PRU strain per mouse

Challenged group^a	Brain cysts per mouse (mean ± SD)^b
PBS	3042.43 ± 115.17
alum	2834.45 ± 100.89
MPLA	2767.36 ± 103.45
alum-MPLA	2532.56 ± 99.45
pEGFP-toxofilin	2048.79 ± 87.44^*
alum-toxofilin	1764.17 ± 78.45^*
MPLA-toxofilin	1563.42 ± 80.48^*
alum-MPLA-toxofiln	1034.49 ± 45.87^*#

^a10 mice from each group were challenged intragastrically by 20 cysts 2 weeks after the last immunization. All samples were run for three times
^bThe mean number of cysts of each group was obtained from every mice brain cysts in the group
^*Compared with PBS, alum, MPLA, alum-MPLA, P < 0.05
^#Compared with pEGFP- toxofilin, alum-toxofilin and MPLA-toxofilin, P < 0.05

ISSN: 1471-2334