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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Vaccination with toxofilin DNA in combination with an alum-monophosphoryl lipid A mixed adjuvant induces significant protective immunity against Toxoplasma gondii

Fig. 4

Green fluorescent microscopy image of HEK293T cells and western blotting. (A1) HEK293 cells transfected with pEGFP-Tgtoxofilin under blue light. (A2) HEK293 cells transfected with pEGFP. (A3) non-transfected HEK293 cells. (B) Western blot analysis of pEGFP-toxofilin protein in transfected HEK293 cells, PET-30 toxofilin protein in bacterial, respectively. (B1) In HEK 293 cells, the expressed proteins were reacted with a T. gondii antibody (lanes 2) and the control empty pEGFP plasmid-transfected cells did not show any band with a T. gondii antibody (lane 1). (B2) PET-30 toxofilin protein were reacted with a T. gondii antibody (lane 4), the empty pET30a vector showed no specific bands with a T. gondii antibody (lane 3). (lane M) Respresents protein molecular weight markers

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BMC Infectious Diseases

ISSN: 1471-2334

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