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Table 2 Cytokine production by splenocytea cultures from immunized BALB/c mice

From: Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice

Group

Cytokine production (pg/mL) b

IFN-γ

IL-2

IL-12

PBS

48.9 ± 6.5

33.2 ± 3.56

37.5 ± 7.3

pEGFP-C1

51.1 ± 7.1

33.9 ± 5.3

38.1 ± 6.0

pSAG1

613.9 ± 59.3*

247.9 ± 29.0*

160.2 ± 35.5*

pROM4

531.8 ± 63.3*

212.4 ± 22.4*

146.1 ± 31.1*

peptide

488.6 ± 79.1*

210.1 ± 20.7*

132.9 ± 26.0*

pROM4/peptide

778.2 ± 93.2*# &

343.2 ± 22.4*# &

247.1 ± 48.8*# &

Group

Cytokine production (pg/mL) b

 

IL-4

IL-10

 

PBS

38.9 ± 8.9

39.5 ± 8.5

 

pEGFP-C1

38.6 ± 10.7

41.5 ± 6.8

 

pSAG1

36.5 ± 4.0

39.7 ± 5.9

 

pROM4

40.2 ± 5.8

40.7 ± 4.8

 

peptide

38.1 ± 7.7

37.6 ± 8.6

 

pROM4/peptide

41.2 ± 5.7

42.4 ± 6.5

 
  1. a Splenocytes from seven mice per group 2 weeks after the final immunization. All samples were performed four times
  2. b Values for IL-12 (p70) and IFN-γ at 96 h, IL-2 and IL-4 at 24 h, IL-10 at 72 h are expressed as mean ± SD
  3. * P < 0.05, as compared with PBS and pEGFP-C1; # P < 0.05, as compared with pROM4 or peptide; & P < 0.05, as compared with pSAG1
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BMC Infectious Diseases

ISSN: 1471-2334

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