Fig. 5

1% Agarose gel containing RFLP profile (vir type) of XhoI digested PCR products. Genomic DNA was prepared using the phenol-chloroform method. vir regulon specific amplicon was amplified by VUF and SBR primers and resolved on 0.8% agarose gel. RFLP was performed using XhoI restriction enzyme and the products were resolved on 1% agarose gel. Lane 1 = 10 kb Super mix marker; Lanes 2-7 = VTi- VTvi