Fig. 4

1% Agarose gel containing RFLP profile (vir type) of HinfI digested PCR products. Genomic DNA was prepared using the phenol-chloroform method. vir regulon specific amplicon was amplified by VUF and SBR primers and resolved on 0.8% agarose gel. RFLP was performed using HinfI restriction enzyme and the products were resolved on 1% agarose gel. Lane 1 = 10 kb Super mix marker; Lanes 2-10 = VT₁ - VT₉ ; Lane11 = 100 bp marker (a). Lane 1 = 10 kb Super mix marker; Lanes 2-13 = VT₁₀ -VT₂₁; Lane 14 = 100 bp marker (b)