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Table 1 Diagnostic data from 3 patients with ZIKV at INMI, Rome [1720]

From: Three cases of Zika virus imported in Italy: need for a clinical awareness and evidence-based knowledge

  

Patient 1

Patient 2

Patient 3

  

At diagnosis (7)*

At day 32 (39)*

At diagnosis (2)*

At day 12 (14)*

At diagnosis (3)*

Ad day 7 (11)*

ZIKV

RT-PCR seruma

Negative

Not performed

Positive

Not performed

Positive

Negative

RT-PCR urinea

Negative

Not performed

Positive

Not performed

Positive

Positive

IgMb

1:40

1:160

1:40

1:640

<1:20

1:80

IgGb

1:80

1:320

<1:20

> = 1:640

<1:20

1:160

 

NTg

<1:20

1:160

Not Performed

<1:20

Not Performed

1:40

Dengue Virus

RT-PCR serumc

Negative

Not performed

Negative

Not performed

Negative

Not performed

RT-PCR urinec

Not performed

Not performed

Negative

Not performed

Negative

Not performed

IgMd

<1:20

Not performed

<1:20

Not performed

<1:20

Not performed

IgGd

<1:20

Not performed

<1:20

Not performed

1:20

Not performed

CHIKV

RT-PCR serume

Negative

Not performed

Negative

Not performed

Negative

Not performed

IgMf

<1:20

Not performed

<1:20

Not performed

<1:20

Not performed

IgGf

<1:20

Not performed

<1:20

Not performed

<1:20

Not performed

  1. *Numbers in brackets indicate days from symptoms onset
  2. apan-flavivirus NS5 nested RT-PCR (modified from [17]), followed by the amplicons’ sequencing and phylogenetic analyses
  3. b: IgG and IgM in house indirect immunofluorescence assay performed with homemade slides spotted with a mix (1:1) of uninfected and ZIKV (MR766 strain)–infected Vero E6 cells, according to [18]. IFA titres <1:20 were considered negative
  4. c: CDC DENV-1-4 Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus (http://www.cdc.gov/dengue/clinicalLab/realTime.html)
  5. d: IgG and IgM indirect immunofluorescence assay Euroimmun Flavivirus Mosaic 2 slides. IFA titres <1:20 were considered negative
  6. e: Real-time RT-PCR targeting the E1 structural protein [19]
  7. f: IgG and IgM indirect immunofluorescence assay Euroimmun Chikungunya virus slides. IFA titres <1:20 were considered negative
  8. g: Neutralisation test (NT) titres <1:20 were considered negative [20]