| |
Patient 1
|
Patient 2
|
Patient 3
|
---|
| |
At diagnosis (7)*
|
At day 32 (39)*
|
At diagnosis (2)*
|
At day 12 (14)*
|
At diagnosis (3)*
|
Ad day 7 (11)*
|
---|
ZIKV
|
RT-PCR seruma
|
Negative
|
Not performed
|
Positive
|
Not performed
|
Positive
|
Negative
|
RT-PCR urinea
|
Negative
|
Not performed
|
Positive
|
Not performed
|
Positive
|
Positive
|
IgMb
|
1:40
|
1:160
|
1:40
|
1:640
|
<1:20
|
1:80
|
IgGb
|
1:80
|
1:320
|
<1:20
|
> = 1:640
|
<1:20
|
1:160
|
|
NTg
|
<1:20
|
1:160
|
Not Performed
|
<1:20
|
Not Performed
|
1:40
|
Dengue Virus
|
RT-PCR serumc
|
Negative
|
Not performed
|
Negative
|
Not performed
|
Negative
|
Not performed
|
RT-PCR urinec
|
Not performed
|
Not performed
|
Negative
|
Not performed
|
Negative
|
Not performed
|
IgMd
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
IgGd
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
1:20
|
Not performed
|
CHIKV
|
RT-PCR serume
|
Negative
|
Not performed
|
Negative
|
Not performed
|
Negative
|
Not performed
|
IgMf
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
IgGf
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
<1:20
|
Not performed
|
- *Numbers in brackets indicate days from symptoms onset
- apan-flavivirus NS5 nested RT-PCR (modified from [17]), followed by the amplicons’ sequencing and phylogenetic analyses
- b: IgG and IgM in house indirect immunofluorescence assay performed with homemade slides spotted with a mix (1:1) of uninfected and ZIKV (MR766 strain)–infected Vero E6 cells, according to [18]. IFA titres <1:20 were considered negative
- c: CDC DENV-1-4 Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus (http://www.cdc.gov/dengue/clinicalLab/realTime.html)
- d: IgG and IgM indirect immunofluorescence assay Euroimmun Flavivirus Mosaic 2 slides. IFA titres <1:20 were considered negative
- e: Real-time RT-PCR targeting the E1 structural protein [19]
- f: IgG and IgM indirect immunofluorescence assay Euroimmun Chikungunya virus slides. IFA titres <1:20 were considered negative
- g: Neutralisation test (NT) titres <1:20 were considered negative [20]