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Table 1 Diagnostic data from 3 patients with ZIKV at INMI, Rome [1720]

From: Three cases of Zika virus imported in Italy: need for a clinical awareness and evidence-based knowledge

   Patient 1 Patient 2 Patient 3
   At diagnosis (7)* At day 32 (39)* At diagnosis (2)* At day 12 (14)* At diagnosis (3)* Ad day 7 (11)*
ZIKV RT-PCR seruma Negative Not performed Positive Not performed Positive Negative
RT-PCR urinea Negative Not performed Positive Not performed Positive Positive
IgMb 1:40 1:160 1:40 1:640 <1:20 1:80
IgGb 1:80 1:320 <1:20 > = 1:640 <1:20 1:160
  NTg <1:20 1:160 Not Performed <1:20 Not Performed 1:40
Dengue Virus RT-PCR serumc Negative Not performed Negative Not performed Negative Not performed
RT-PCR urinec Not performed Not performed Negative Not performed Negative Not performed
IgMd <1:20 Not performed <1:20 Not performed <1:20 Not performed
IgGd <1:20 Not performed <1:20 Not performed 1:20 Not performed
CHIKV RT-PCR serume Negative Not performed Negative Not performed Negative Not performed
IgMf <1:20 Not performed <1:20 Not performed <1:20 Not performed
IgGf <1:20 Not performed <1:20 Not performed <1:20 Not performed
  1. *Numbers in brackets indicate days from symptoms onset
  2. apan-flavivirus NS5 nested RT-PCR (modified from [17]), followed by the amplicons’ sequencing and phylogenetic analyses
  3. b: IgG and IgM in house indirect immunofluorescence assay performed with homemade slides spotted with a mix (1:1) of uninfected and ZIKV (MR766 strain)–infected Vero E6 cells, according to [18]. IFA titres <1:20 were considered negative
  4. c: CDC DENV-1-4 Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus (http://www.cdc.gov/dengue/clinicalLab/realTime.html)
  5. d: IgG and IgM indirect immunofluorescence assay Euroimmun Flavivirus Mosaic 2 slides. IFA titres <1:20 were considered negative
  6. e: Real-time RT-PCR targeting the E1 structural protein [19]
  7. f: IgG and IgM indirect immunofluorescence assay Euroimmun Chikungunya virus slides. IFA titres <1:20 were considered negative
  8. g: Neutralisation test (NT) titres <1:20 were considered negative [20]